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Novel application of Jingzhaotoxin
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A technology of tassels spider and toxin, which is applied in the field of application of tassels spider toxin
Active Publication Date: 2013-03-06
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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The effective treatment time for cerebral apoplexy is only 6 hours or less after the onset of stroke. If the embolus cannot be effectively opened or the hemorrhage can be cleared within this period, the brain damage will be irreversible. need 20 hours
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Embodiment 1
[0042] Embodiment 1, the in vitro thrombolytic activity of Jingzhao's toxin
[0043] 1. Preparation of fibrin plates
[0044] 1. Dilute 0.04g of human fibrinogen into 10ml of sterilized PBS buffer so that the final concentration is 4mg / ml, which is the fibrinogen solution.
[0045] 2. Dilute thrombin with 1ml of PBS buffer to a final concentration of 1mg / ml, which is the thrombin solution.
[0046] 3. Dissolve 0.1g of agarose in 10ml of PBS buffer and heat to dissolve, which is the agarose solution.
[0047]4. Add 1ml of thrombin solution to the wall into 10ml of fibrinogen solution, mix quickly and pour into 10ml of agarose solution melted at 37°C, mix immediately and pour into a disposable petri dish with a diameter of 10.5cm medium; place at room temperature for 30 minutes, transfer to a 4°C refrigerator and place for 30 minutes to obtain a fibrin plate; use a 100 μl tip to punch holes on the fibrin plate; irradiate for 10 minutes under ultraviolet light.
[0048] 2. Det...
Embodiment 3
[0063] Embodiment 3, the in vivo activity test of Jingzhao's toxin
[0064] 1. Establishment of a mouse cerebral ischemia-reperfusion model
[0065] Construct a mouse cerebral ischemia-reperfusion model (also known as a mouse middle cerebral artery occlusion model or MCAO model; the construction method was established by Longa et al. in 1989, and it is a classic method for studying cerebral ischemia at present; references: LONGA, E.Z., et al. al., REVERSIBLE MIDDLE CEREBRAL-ARTERY OCCLUSION WITHOUT CRANIECTOMY IN RATS. STROKE, 1989.20(1): p.84-91.), the steps are as follows:
[0066] (1) Anesthesia
[0067] Male Kunming mice (body weight 20-30 g, purchased from Guangzhou Experimental Medicine Animal Center) were injected with 10% (volume percentage) chloral hydrate aqueous solution, and the injection dose was 300 mg chloral hydrate / kg mouse body weight, intravenously.
[0068] (2) MCAO modeling
[0069] After 10 minutes of anesthesia in step (1), the initial perfusion was c...
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Abstract
The invention discloses novel applications of Jingzhaotoxin, and particularly relates to an application of Jingzhaotoxin in a medicament for treating and / or preventing cerebrovascular disorder (apoplexy) and an application of Jingzhaotoxin in a medicament for preventing and / or treating nerve injuries. According to the invention, the Jingzhaotoxin has a remarkable repairing effect on nerve injuries, such as anticoagulation, free radical removal [superoxide dismutase (SOD) activity reinforcement, malondialdehyde (MDA) level reduction, inflammation cyclooxygenase-2 (COX-2) transcriptional level reduction and other applications are included], ischemic stroke symptom relief, cerebral infarction area reduction and the like.
Description
technical field [0001] The present invention relates to a new application of Jingzhao's toxin, in particular to the application of Jingzhao's toxin in the treatment and / or prevention of stroke (stroke), and the application of Jingzhao's toxin in the preparation of preventive And / or the application in the medicine of treatment nerve injury. Background technique [0002] With the acceleration of population aging worldwide, the incidence of stroke is increasing year by year. In recent years, stroke has become the first cause of disability and the second cause of death worldwide. According to the World Health Organization's MONICA project survey, the average incidence of stroke in the East is higher than in the West, and the incidence of stroke in Asian countries such as Japan is at the top of the world; at the same time, the plan has monitored about 5 million people in my country for 8 years. It shows that the incidence rate of stroke in my country is 250 / 100,000, second only ...
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Application Information
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