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Paecilomycescicadae (Miq.)Samson and application thereof

A technology of Paecilomyces cicadae strains and a preservation number, applied in the field of microorganisms, can solve the problems of limited wild Cordyceps sinensis resources, destruction of vegetation and ecological environment, high cost, etc., and achieves the effects of extensive industrial application value, improvement of kidney function, and high yield.

Active Publication Date: 2014-06-18
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The resources of wild Cordyceps sinensis are limited. Over the years, due to its high value, it has formed indiscriminate harvesting, which has caused serious damage to vegetation and the ecological environment.

Method used

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  • Paecilomycescicadae (Miq.)Samson and application thereof
  • Paecilomycescicadae (Miq.)Samson and application thereof
  • Paecilomycescicadae (Miq.)Samson and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the isolation of Paecilomyces cicadae (Miq.) Samson] CGMCC No.3453 of cicada Paecilomyces strain

[0036] 1. Prepare the medium according to the following ratio;

[0037] Potato sucrose agar medium: 200g potato, 20g sucrose, 20g agar, 1000ml water;

[0038] Peptone sucrose agar medium: 20g peptone, 10g sucrose, 20g agar, 1000ml water.

[0039] 2. Separation of strains:

[0040] Collect pure and pollution-free wild cicada specimens from the source of Sanjiang, Yunnan, and use flame-sterilized transplant forceps on the purification workbench to take a small amount of conidia from the samples and culture them on the PSA plate added with Bengal red at 22°C-25°C After 120 hours, a single colony was picked and inserted into the slant medium, cultured at 22°C-25°C and purified repeatedly to obtain the Paecilomyces cicadae (Miq.) Samson of the present invention.

[0041] 3. Strain culture:

[0042] Inoculate Paecilomyces cicadae (Miq.) Samson] CGMCC No.3453 on p...

Embodiment 2

[0051] Example 2 Paecilomyces cicadae (Miq.) Samson] CGMCC No.3453 Solid Culture and Composition Analysis

[0052] Solid culture of bacteria:

[0053] (1) bacterial classification preparation: the bacterial classification gained in embodiment 1;

[0054] (2) Batching box: prepare solid medium, pack the prepared medium into the culture box, the charging amount is 15%, and seal it with parafilm; the solid medium proportioning described in this embodiment is matrix 46 Parts by weight, 10 parts by weight of bagasse, 0.5 parts by weight of shell powder, 5 parts by weight of silkworm chrysalis powder, 0.5 parts by weight of potassium nitrate, and 44 parts by weight of water; The weight percentage of sucrose is 98%, and the weight percentage of sucrose is 2%). The preparation method is as follows: first boil millet, dissolve sucrose and potassium nitrate in water, and then mix uniformly with bagasse, shell powder and silkworm chrysalis powder in proportion.

[0055] (3) Sterilizat...

Embodiment 3

[0137] Embodiment 3, the influence of Paecilomyces cicadae culture on the renal function of 5 / 6 nephrectomy rat model

[0138] Test object: the culture of Example 2 (hereinafter referred to as artificial cicadae).

[0139] Positive control drug: Kesuya, produced by Hangzhou Merck Pharmaceutical Co., Ltd.; natural cicadae, produced in Zhejiang.

[0140] Experimental animals:

[0141] Seventy SPF male SD rats, 2 months old, weighing 200±10g, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0142] Grouping and Modeling:

[0143] After one week of adaptive feeding, SD rats were randomly divided into two groups: blank group (sham operation group) with 8 rats and experimental group (operation group) with 62 rats. The experimental rat model was established by the method of 5 / 6 major nephrectomy: in the first operation, the weight of the total left nephrectomy renal cortex accounted for 2 / 3 of the weight of the left kidney in...

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Abstract

The invention belongs to the field of microbes, and in particular relates to a Paecilomycescicadae (Miq.)Samson and application thereof. The Paecilomycescicadae (Miq.)Samson has the collection number of CGMCCNo.3453. The Paecilomycescicadae (Miq.)Samson with the collection number of CGMCCNo.3453 has the characteristics of simple culture and high yield; and a culture product has effects of resisting tumors, regulating immunity, reducing blood pressure, blood sugar and blood fat, improving eyesight, resisting radiation, clearing away heat and easing pain, calming and promoting sleep, nourishing and strengthening, improving kidney function and the like, so the invention has high industrial application value.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Paecilomyces cicadae and an application thereof. Background technique [0002] Cicada flower, also known as insect flower, is the product of some cicada nymphs parasitized by Paecilomyces cicadae, and it is a complex of bacteria and insects. This fungus was named by Miquel in 1838 as Isaria cicadae. Since then, there have been many synonyms of the same thing. Such as Cordyceps cicadae, Isaria basili, Sphaeria sinclairii, Torrubia caespitosa, Cordyceps sinclairii, Isariahariottii, Cordyceps sobolifera, Sinclair Isaria sinclairii, Isaria mokanshawii and Isaria arbuscula, etc. [0003] The sexual stage of Paecilomyces cicadae is considered to be Cordyceps cicadae, Paecilomyces cicadae is widely distributed in nature, and Paecilomyces cicadae is rare. What is often collected is the parasitic complex of Paecilomyces cicadae and its host mountain cicada nymph. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23L1/29A23L1/28A61K36/06A61K8/99A61P35/00A61P37/02A61P3/10A61P3/06A61P9/12A61P27/02A61P17/16A61P29/00A61P25/20A61P13/12C12R1/79A23L33/00
Inventor 陈祝安孙长胜赵伟李成
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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