Culture method and culture medium of cellar mud bacterium liquid with special flavor-producing function
A culture method and technology of functional bacteria, applied in the field of winemaking, can solve problems such as lack of research
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Embodiment 1
[0026] Medium formula: Extraction liquid of discarded grains: 5%, high-quality pit mud: 8%, infusion liquid of bottom grains: 10%, K 2 HPO 4 : 0.2%, NaAC: 0.1%, MgSO 4 : 0.005%, 95% alcohol: 4%, yellow water: 6%, water: 66.695%.
[0027] The high-quality pit mud in the above formula is pit mud that has been used for more than 20 years. The relevant indicators are: moisture (%): 39; pH value: 6.5; caproic acid bacteria (×10 7 per g. dry soil): 2.0; Methanogens (×10 3 per g. dry soil): 4.5; nitrate-reducing bacteria (×10 5 per g. dry soil): 2.8; ammoniacal nitrogen (mg / 100g. dry soil): 296; available phosphorus (mg / 100g. dry soil): 164; humus (%): 12.
[0028] The method for cultivating the special aroma-generating functional bacterial liquid of pit mud by using the above-mentioned medium comprises the following steps: A. Check the fermenter to ensure that there is no water leakage, electric leakage, or steam leakage. Clean the fermenter, then close the bottom valve and ...
Embodiment 2
[0030] Medium formula: Extraction liquid of discarded grains: 3%, high-quality pit mud: 9.19%, infusion liquid of bottom grains: 10%, K 2 HPO 4 : 0.5%, NaAC: 0.3%, MgSO 4 : 0.01%, 95% alcohol: 2%, yellow water: 5%, water: 70%.
[0031] The high-quality pit mud in the above formula is pit mud that has been used for more than 20 years. The relevant indicators are: moisture (%): 40; pH value: 6.2; caproic acid bacteria (×10 7 per g. dry soil): 1.8; Methanogens (×10 3 per g. dry soil): 4.2; nitrate-reducing bacteria (×10 5 per g. dry soil): 2.5; ammoniacal nitrogen (mg / 100g. dry soil): 285; available phosphorus (mg / 100g. dry soil): 180; humus (%): 12.5.
[0032] The method for cultivating the special aroma-generating functional bacterial liquid of pit mud by using the above-mentioned medium comprises the following steps:A. Check the fermenter to ensure that there is no water leakage, electric leakage, or steam leakage. Clean the fermenter, then close the bottom valve and t...
Embodiment 3
[0034] Medium formula: Extraction liquid of discarded grains: 4.8%, high-quality pit mud: 10%, infusion liquid of bottom grains: 10%, K 2 HPO 4 : 0.4%, NaAC: 0.2%, MgSO 4 : 0.08%, 95% alcohol: 3.52%, yellow water: 10%, water: 61%.
[0035] The high-quality pit mud in the above formula is pit mud that has been used for more than 20 years. The relevant indicators are: moisture (%): 39.5; pH value: 6.1; caproic acid bacteria (×10 7 per g. dry soil): 1.9; Methanogens (×10 3 per g. dry soil): 4.4; nitrate-reducing bacteria (×10 5 per g. dry soil): 2.7; ammoniacal nitrogen (mg / 100g. dry soil): 290; available phosphorus (mg / 100g. dry soil): 178; humus (%): 13.
[0036] The method for cultivating the special aroma-generating functional bacterial liquid of pit mud by using the above-mentioned medium comprises the following steps: A. Check the fermenter to ensure that there is no water leakage, electric leakage, or steam leakage. Clean the fermenter, then close the bottom valve ...
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