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Application of transcription factor FOXO1 in regulation of specific micro-ribonucleic acid (RNA) miR-122 of human liver

A technology of rnamir-122 and transcription factor, which is applied in the field of expression regulation of small RNA by transcription factor and eukaryotic gene expression regulation, and can solve the problems that the expression and function of miR-122 have not been reported.

Inactive Publication Date: 2012-01-11
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

[0005] However, there is no report on whether FOXO1 is related to the expression and function of miR-122

Method used

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  • Application of transcription factor FOXO1 in regulation of specific micro-ribonucleic acid (RNA) miR-122 of human liver
  • Application of transcription factor FOXO1 in regulation of specific micro-ribonucleic acid (RNA) miR-122 of human liver
  • Application of transcription factor FOXO1 in regulation of specific micro-ribonucleic acid (RNA) miR-122 of human liver

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Embodiment Construction

[0030] 1. Using bioinformatics tools to analyze the potential binding sites of FOXO1 in the core promoter region of miR-122

[0031] Before using experimental methods to verify whether FOXO1 binds to the core promoter region of miR-122 and regulates its transcription, first use the relatively mature online tools for predicting transcription factor binding sites, such as UCSC, ECR Browser, etc. to miR-122 The core promoter region is predicted, and the focus is appropriately narrowed before experimental verification.

[0032] 1.1 UCSC Genome Bioinformatics Analysis

[0033] For the 5kb regulatory region upstream of miR-122, use the online tool UCSC GenomeBrowser http: / / genome.ucsc.edu / Prediction of potential binding sites for FOXO1. Such as figure 1 As shown, there are two potential regulatory sites of FOXO1, one is remote regulatory site I (site I) 4870bp upstream of the mature version of miR-122 ( chr18: 54264417-54264430 ), the other is the proximal regulatory site II ...

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Abstract

The invention relates to application of a transcription factor forkhead box protein O1 (FOXO1) in regulation of specific micro-ribonucleic acid (RNA) miR-122. Experimental data of the inventor prove that: the FOXO1 can be used as a new regulating factor for the miR-122, and regulates the transcription of a primary transcript of the miR-122 by an interferon-responsive element (IRE) locus bound to a core promoter area of the miR-122, so that the FOXO1 can participate in the regulation of functions of the miR-122. The regulation is influenced by signal path closely related to a nutrition state, as well as the competition of a hepatocyte nuclear factor 4 (HNF4) for the binding of the locus, and the transcription factor FOXO1 can finally participate in the fine regulating process of lipid metabolism.

Description

technical field [0001] The invention relates to the field of eukaryotic gene expression regulation in medical molecular biology research. Specifically, the present invention relates to the field of expression regulation of small RNAs by transcription factors. Background technique [0002] microRNA (miRNA) is a kind of non-coding single-stranded small molecule RNA with a length of about 20-24nt. They cause the mRNA to The translation of the molecule is inhibited, thereby regulating downstream biological processes. [0003] miR-122 is a human liver-specific small RNA. Its initial transcript is derived from the splicing of the hcr gene transcript and is located on human chromosome 18 18q21.31. miR-122 is continuously expressed during liver development and is highly conserved during evolution. Studies have shown that: under pathological conditions, miR-122 promotes the replication of hepatitis C virus (HCV) in liver cells, and miR-122 is closely related to the occurrence, de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P1/16
Inventor 刘德培习杨李振亚曾超朱文楠吕湘
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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