Fermentation and preparation of composite microbial agent for degrading sulfonylurea herbicide with high efficiency
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A technology of compound microbial inoculants and compound inoculants, applied in the directions of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of accumulation of herbicide residues, affecting the growth of subsequent crops, and phytotoxicity of subsequent crops.
Inactive Publication Date: 2012-01-18
HEBEI AGRICULTURAL UNIV.
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[0003] Since the advent of sulfonylurea herbicides, they have made great contributions to the world's agricultural production. Due to their extremely high residual activity and selectivity, some difficulties have been encountered during their use, the most prominent being the impact on the aftermath. The long-term residual phytotoxicity of stubble crops, because the migration and leaching of this type of herbicide is weak, when its residue reaches a certain dose, it will cause phytotoxicity to subsequent crops
Some fields where long-term residual herbicides have been used for a long time also have accumulated herbicide residues, which seriously affects the growth of subsequent crops, forming the phenomenon of "cancer fields"
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example 1
[0030] Example 1, the prepared bacterial agent is diluted to 1000 times, get 1ml, join in the 100ml test water sample, the initial concentration of sulfuron-methyl in the test water sample is 2.3mg / L, after 72 hours, the water sample The residual concentration of metsulfuron-methyl in the drug was lower than the detection limit, that is, no residual nicosulfuron-methyl could be detected.
example 2
[0031] Example 2, the prepared bacterium agent is diluted to 1000 times, get 10ml, evenly spray and mix and join in 1kg for testing upper soil sample, the residual amount of tribenuron-methyl in the test soil is 2.0mg / kg, 72 Hours later, the residual amount of tribenuron-methyl in the soil sample was lower than the detection limit, that is, no residual tribenuron-methyl could be detected.
example 3
[0032] Example 3, get prepared bacterium agent 10kg, apply to the field for testing of one mu of land along with irrigation water, the initial residual amount of nicosulfuron in the upper soil of the field for testing is 1.6mg / kg, 72 hours The residual amount of nicosulfuron in the soil of the test field was lower than the detection limit, that is, no residual nicosulfuron could be detected.
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Abstract
The invention relates to a fermentation and preparation method of a composite microbial agent for degrading sulfonylurea herbicides in soil and water with high efficiency, and the composite microbial agent is fermented compositely by using bacillus benzoevorans ACCC 10244 and bacillus chitinivorous ACCC 10227 as bacterial sources. After fermentation, a carrier substance of alginic acid (in a form of micro-colloid in aqueous solutions), a electrolyte (potassium nitrate) and an antifreeze agent (glycerin) are added into the fermented bacterial liquid; the bacterial number ratio of bacillus benzoevorans ACCC 10244 and bacillus chitinivorous ACCC 10227 in the prepared bacterial agent is 1:1, and the bacterial number contents of both bacteria in the bacterial agent are up to above 1 billion / ml. The bacterial agent can degrade the followings with high efficiency: nicosulfuron, suifometuron-methyl, monosulfuron, bensulfuron-methyl, thifensulfuron-methyl, tribenuron-methyl, metsulfuron-methyl, chlorsulfuron, methyl sulfuron, etc.
Description
technical field [0001] The invention relates to the fermentation and preparation of a compound microbial bacterial agent, which can efficiently degrade sulfonylurea herbicides in soil and water, such as: nicosulfuron, sulfuron-methyl, monosulfuron-methyl, benzyl rimsulfuron-methyl, thifensulfuron-methyl, tribenuron-methyl, metsulfuron-methyl, chlorsulfuron-methyl, methylsulfuron-methyl, etc. technical background [0002] Sulfonylurea herbicides are currently the most widely used herbicides in the world. It was successfully researched by DuPont in the 1970s, and the wheat field herbicide chlorsulfuron was first developed in 1982, and later developed Many high-efficiency, broad-spectrum, low-toxicity, high-selectivity sulfonylurea herbicides have been produced. At present, the sulfonylurea herbicides widely used in my country mainly include pyrazosulfuron-methyl, nicosulfuron-methyl, ethametsulfuron-methyl, tribenuron-methyl, chlorimuron-methyl, bensulfuron-methyl, etc., whic...
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