Fermentation and preparation of composite microbial agent for degrading sulfonylurea herbicide with high efficiency

A compound microbial inoculant and compound inoculant technology, applied in the directions of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of weak migration and leaching of herbicides, long residual phytotoxicity of subsequent crops, affecting the growth of subsequent crops, etc.

A compound microbial inoculant and compound inoculant technology, applied in the directions of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of weak migration and leaching of herbicides, long residual phytotoxicity of subsequent crops, affecting the growth of subsequent crops, etc.

CN102321539BInactive Publication Date: 2013-05-29HEBEI AGRICULTURAL UNIV.
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  • Fermentation and preparation of composite microbial agent for degrading sulfonylurea herbicide with high efficiency
  • Fermentation and preparation of composite microbial agent for degrading sulfonylurea herbicide with high efficiency

Examples

Experimental program
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Effect test

example 1

[0030] Example 1, the prepared bacterial agent is diluted to 1000 times, get 1ml, join in the 100ml test water sample, the initial concentration of sulfuron-methyl in the test water sample is 2.3mg / L, after 72 hours, the water sample The residual concentration of metsulfuron-methyl in the drug was lower than the detection limit, that is, no residual nicosulfuron-methyl could be detected.

example 2

[0031] Example 2, the prepared bacterium agent is diluted to 1000 times, get 10ml, evenly spray and mix and join in 1kg for testing upper soil sample, the residual amount of tribenuron-methyl in the test soil is 2.0mg / kg, 72 Hours later, the residual amount of tribenuron-methyl in the soil sample was lower than the detection limit, that is, no residual tribenuron-methyl could be detected.

example 3

[0032] Example 3, get prepared bacterial agent 10kg, apply to the field for testing of one mu of land along with irrigation water, the initial residual amount of nicosulfuron in the upper soil of the field for testing is 1.6mg / kg, 72 hours The residual amount of nicosulfuron in the soil of the test field was lower than the detection limit, that is, no residual nicosulfuron could be detected.

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Abstract

The invention relates to a fermentation and preparation method of a composite microbial agent for degrading sulfonylurea herbicides in soil and water with high efficiency, and the composite microbial agent is fermented compositely by using bacillus benzoevorans ACCC 10244 and bacillus chitinivorous ACCC 10227 as bacterial sources. After fermentation, a carrier substance of alginic acid (in a form of micro-colloid in aqueous solutions), a electrolyte (potassium nitrate) and an antifreeze agent (glycerin) are added into the fermented bacterial liquid; the bacterial number ratio of bacillus benzoevorans ACCC 10244 and bacillus chitinivorous ACCC 10227 in the prepared bacterial agent is 1:1, and the bacterial number contents of both bacteria in the bacterial agent are up to above 1 billion / ml. The bacterial agent can degrade the followings with high efficiency: nicosulfuron, suifometuron-methyl, monosulfuron, bensulfuron-methyl, thifensulfuron-methyl, tribenuron-methyl, metsulfuron-methyl, chlorsulfuron, methyl sulfuron, etc.

Description

technical field [0001] The invention relates to the fermentation and preparation of a compound microbial bacterial agent, which can efficiently degrade sulfonylurea herbicides in soil and water, such as: nicosulfuron, sulfuron-methyl, monosulfuron-methyl, benzyl rimsulfuron-methyl, thifensulfuron-methyl, tribenuron-methyl, metsulfuron-methyl, chlorsulfuron-methyl, methylsulfuron-methyl, etc. technical background [0002] Sulfonylurea herbicides are currently the most widely used herbicides in the world. It was successfully researched by DuPont in the 1970s, and the wheat field herbicide chlorsulfuron was first developed in 1982, and later developed Many high-efficiency, broad-spectrum, low-toxicity, high-selectivity sulfonylurea herbicides have been produced. At present, the sulfonylurea herbicides widely used in my country mainly include pyrazosulfuron-methyl, nicosulfuron-methyl, ethametsulfuron-methyl, tribenuron-methyl, chlorimuron-methyl, bensulfuron-methyl, etc., whic...

Claims

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Application Information

Patent Timeline
29 May 2013
Publication
CN102321539B
IPC
C12N1/00; C12N1/20; A62D3/02; C02F3/34; C12R1/07; A62D101/04; A62D101/28
Inventors
康占海; 任成才