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Chinese isolate of Leachiii mycoplasma, isolation medium and purpose thereof

A technology for mycoplasma and isolates, which is applied in the field of mycoplasma isolates to develop and control or detect diseases caused by Mycoplasma Leachii in the field of biological products, so as to achieve the effects of shortening the culture period, reducing production costs and reducing side effects

Inactive Publication Date: 2012-02-08
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0008] So far, no isolates of Mycoplasma Leachii have been isolated in China. Therefore, the isolation of a Mycoplasma Leachii isolate in China is of great importance for the study of the transmission route of Mycoplasma Leachii and the development of biological products for the prevention or treatment of diseases caused by Mycoplasma Leachii significance

Method used

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  • Chinese isolate of Leachiii mycoplasma, isolation medium and purpose thereof
  • Chinese isolate of Leachiii mycoplasma, isolation medium and purpose thereof
  • Chinese isolate of Leachiii mycoplasma, isolation medium and purpose thereof

Examples

Experimental program
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Effect test

Embodiment

[0039] 1. Test materials and methods

[0040] 1. Sample source

[0041] From February to June 2009, more than 300 female calves on a dairy farm in northern China suffered from severe polyarthritis, from which 2 joint fluids of calves with early onset and typical symptoms were aseptically collected, -70 ℃ frozen for later use.

[0042] 2. Test material

[0043] DNA Gel Recovery Kit, Column Small Tissue / Cell Genomic DNA Extraction Kit; Ex Taq DNA Polymerase; Hyclone Donor Horse Serum; PPLO Broth (BD-Difco); Yeast Infusion (BD-Bacto) ; Other chemical reagents used in the experiment were of analytical grade.

[0044] 3. Main instruments and equipment

[0045] Pipettes of various specifications and 5415R, 5810R high-speed desktop low-temperature centrifuges (Eppendorf, Germany); MCO-15AC carbon dioxide incubator, MDF-U32V ultra-low temperature refrigerator (SANYO, Japan); IX51 inverted microscope (OLYMPUS); Safety cabinet (LABCONCO, USA); DK-8D electric heating constant temper...

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Abstract

The invention discloses a Chinese isolate of Leachiii mycoplasma, an isolation medium and purpose thereof. The Chinese isolate of Leachiii mycoplasma has a microbe preservation No. of CGMCC No.3808. The invention also discloses a medium suitable for isolation or subculturing of the Chinese isolate of Leachiii mycoplasma. Pathogenic test results show that inoculating subculture of Leachiii mycoplasma isolate of the invention in joint of 1-2 month healthy calf can replicate typical polyarthritis and cause certain death; the dead calf has obvious constitutional symptom and obvious pathological changes in multiple tissues and organs. According to clinical symptoms and pathological changes of clinical morbidity and artificial inoculated calf, epidemic features of the disease, tracking epidemiology investigation and singular PRC identification and genetic evolution analysis of isolated pathogeny, it is proved that the mycoplasma isolated by the invention is Leachiii mycoplasma.

Description

technical field [0001] The present invention relates to a strain of mycoplasma (Mycoplasma) isolate, relate in particular to the Chinese isolate of Leachii mycoplasma and the culture medium that is suitable for this isolate and subculture, the present invention also relates to this isolate in the research and development of prevention and control or detection by Leachii mycoplasma The use in disease-causing biological products belongs to the field of separation and application of mycoplasma. Background technique [0002] Mycoplasma (Mycoplasma) is a class of minimal prokaryotic microorganisms that lack cell walls, are highly pleomorphic, can pass through bacteria filters, and can grow and reproduce in non-living media. Mycoplasma was first isolated by Nocard et al. in 1898, and was officially named Mycoplasma in 1956. A new independent class - Mollicutes was established in the microbial classification, and it became an independent discipline, that is, mycoplasma. [0003] M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04A61P11/00A61P19/02A61P29/00G01N33/569C12R1/35A61K35/741
Inventor 于力常继涛刘海军
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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