Method for measuring beta-sitosterol content of supercritical extract of pine pollen

A technology of supercritical extract and determination method is applied in the field of determination of the content of beta-sitosterol in supercritical extract of pine pollen, which can solve the problem of few active ingredients, quality control standards of active active ingredients, complex active ingredients of pine pollen, etc. problem, to achieve the effect of good precision and advanced means

Active Publication Date: 2014-04-09
JIANGSU UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The active ingredients of pine pollen are complicated. Although it is recorded in the "Chinese Pharmacopoeia" and stipulates the preparation method, microscopic identification and inspection items, there is no quality control standard for the content of its active active ingredients.
Supercritical extract of pine pollen is a traditional Chinese medicine extract with market prospects, but there are few research literatures on its active ingredients. There are no literatures and patents on the extraction of sterol components, GC-MS identification and determination technology of supercritical extract of pine pollen.

Method used

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  • Method for measuring beta-sitosterol content of supercritical extract of pine pollen
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  • Method for measuring beta-sitosterol content of supercritical extract of pine pollen

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Embodiment Construction

[0019] The present invention will be further described below by specific examples.

[0020] 1. Experimental part

[0021] Instruments and reagents

[0022] 1200L Gas Chromatograph / Mass Spectrometer (Varian, Germany); Liquid Chromatography (Shimadzu, Japan) including: LC-10AD pump, Shimpark C18 column, SPD-20A UV detector, SPD-M10A DAD detector, CLASS- VP workstation; XT-102A electronic balance (Switzerland Precisa), etc.

[0023] Pine Pollen Supercritical CO 2 Extract (SFE-PP, Jiangsu Jiangdayuan Ecological Biotechnology Co., Ltd.); β-sitosterol reference substance (Sigma-Aldrich Company, USA); except for methanol used in high-performance liquid chromatography, which was chromatographically pure, the rest of the reagents were analytically pure ( Sinopharm Chemical Reagent Co., Ltd.).

[0024] experimental method

[0025] Preparation of standard stock solution: Accurately weigh an appropriate amount of β-sitosterol reference substance and put it in a 10 ml measuring bottl...

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Abstract

The invention relates to a method for measuring active substance beta-sitosterol content of a supercritical extract of pine pollen. Sterol components are analyzed and measured by using a gas chromatography-mass spectrometry combined method and a high performance liquid chromatography. The method comprises the following steps of: determining that the main component of sterols in the supercritical extract of the pine pollen is beta-sitosterol by gas chromatography-mass spectrometry combined analysis; performing saponification extraction; and measuring the content of the beta-sitosterol by using the high performance liquid chromatography. A simple and feasible extraction and analysis method for the sterol components in the supercritical extract of the pine pollen is established for the first time; and the method is advanced and good in accuracy and precision, and the quality condition of the supercritical extract of the pine pollen can be evaluated by measuring the content of the beta-sitosterol.

Description

technical field [0001] The invention relates to a method for determining the content of active ingredient β-sitosterol in pine pollen supercritical extract. Background technique [0002] Pine pollen (Pollen Pini) is the light pollen obtained from Pinus massonina lamb, Pinus tabulaefermiscarr and other plants in the spring when the male bulbs are just opened, dried, rubbed pollen, collected, and removed impurities. Yellow fine pollen is a species recorded in "Chinese Pharmacopoeia" [see: ChP (Chinese Pharmacopoeia). 2010. VolⅠ(Part One): 191]. The development and utilization of pine pollen has a long history in our country. The various biologically active substances contained in it have an important regulating effect on the life activities of the human body. a pharmacological effect. Literature reports that pine pollen is rich in phytosterols, mainly composed of β-sitosterol, campesterol and a small amount of stigmasterol. Phytosterols have a significant effect on lowering...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 余江南童姗姗徐希明邓倩
Owner JIANGSU UNIV
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