Detection method for flavone component in clematis filamentosa dunn, and application thereof
A detection method and technology for flavonoids, applied in the field of medicine, can solve the problems of poor specificity of flavonoids and cannot reflect the content of the components, and achieve the effects of standardization, stability and consistency, good specificity and high precision
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Embodiment 1
[0035] Waters 600 high-performance liquid chromatograph, ultraviolet detector, column thermostat; chromatographic column: use octadecylsilane bonded silica gel as filler: XBridge Shield RP18 (4.6mm×250mm, 5μm). Acetonitrile (chromatographically pure, Merck, USA), ultrapure water; other reagents were analytically pure.
[0036]1. Preparation of the test solution: Take 5.0 g of Ganmotong medicinal material powder (passed through No. 3 sieve), weigh it accurately, put it in a stoppered conical flask, add 50 mL of ethanol with a concentration of 80% by volume, weigh it , heated to reflux for 1.5h, allowed to cool, and then weighed, supplemented with 80% ethanol to lose weight, shaken, filtered, the filtrate was concentrated under reduced pressure to no alcohol smell, diluted with water to 15mL, each time adding petroleum ether (60 ~90°C) 15mL for 2 extractions, the petroleum ether solution was discarded; the water layer was further extracted 2 times with 15mL ethyl acetate, the et...
Embodiment 2
[0073] Embodiment 2 Application of detection method of the present invention
[0074] 1, the preparation of tablet (can refer to existing conventional method. For example, according to the Guanxinkang tablet that the extraction process that provides in the drug standard WS3-B-2759-97 issued by the ministry is prepared) desirable Radix Glycyrrhizae is chopped, add water Decoct twice, decoct for 2 hours for the first time, decoct for 1 hour for the second time, filter, combine the filtrate, concentrate to an appropriate amount, add ethanol to make the alcohol content reach 65-70% (volume ratio concentration), let stand , filtered, the filtrate was concentrated to a thick paste, an appropriate amount of auxiliary materials was added, granulated by conventional methods, dried, compressed into tablets, and coated to obtain tablets.
[0075] 2. Take 20 tablets above, remove the sugar coating or film coating, grind the plain tablets finely, weigh about 1.0g of the powder, weigh it ac...
Embodiment 3
[0078] Embodiment 3 Application of detection method of the present invention
[0079] 1. Preparation of tablets: Take the leaves of Glycyrrhiza radicis, chop them, add ethanol to make the alcohol content reach 65-70%, reflux extraction twice, the first time for 2 hours, the second time for 1 hour, filter, combine the filtrates, Concentrate the filtrate to a thick paste (density: 1.3-1.4, measured at 80°C), add appropriate amount of excipients, conventionally granulate, dry, compress into tablets, and coat.
[0080] 2. Take 20 of the above-mentioned tablets, remove the sugar coating or film coating, grind the plain tablets finely, weigh about 1.0 g of the powder, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50 mL of 80% ethanol accurately, and weigh it. Ultrasonic (power 200w, frequency 40kHz) extraction 0.5 ~ 1h, let cool, and then weighed, with 80% ethanol to make up for the lost weight, shake well, filter, the filtrate is concentrated under reduced pressu...
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