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Detection method for flavone component in clematis filamentosa dunn, and application thereof

A detection method and technology for flavonoids, applied in the field of medicine, can solve the problems of poor specificity of flavonoids and cannot reflect the content of the components, and achieve the effects of standardization, stability and consistency, good specificity and high precision

Active Publication Date: 2014-11-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this detection method using ultraviolet spectrophotometry to detect flavonoids in Ganmotong has poor specificity and cannot reflect the content of specific flavonoids in this medicinal material.

Method used

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  • Detection method for flavone component in clematis filamentosa dunn, and application thereof
  • Detection method for flavone component in clematis filamentosa dunn, and application thereof
  • Detection method for flavone component in clematis filamentosa dunn, and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Waters 600 high-performance liquid chromatograph, ultraviolet detector, column thermostat; chromatographic column: use octadecylsilane bonded silica gel as filler: XBridge Shield RP18 (4.6mm×250mm, 5μm). Acetonitrile (chromatographically pure, Merck, USA), ultrapure water; other reagents were analytically pure.

[0036]1. Preparation of the test solution: Take 5.0 g of Ganmotong medicinal material powder (passed through No. 3 sieve), weigh it accurately, put it in a stoppered conical flask, add 50 mL of ethanol with a concentration of 80% by volume, weigh it , heated to reflux for 1.5h, allowed to cool, and then weighed, supplemented with 80% ethanol to lose weight, shaken, filtered, the filtrate was concentrated under reduced pressure to no alcohol smell, diluted with water to 15mL, each time adding petroleum ether (60 ~90°C) 15mL for 2 extractions, the petroleum ether solution was discarded; the water layer was further extracted 2 times with 15mL ethyl acetate, the et...

Embodiment 2

[0073] Embodiment 2 Application of detection method of the present invention

[0074] 1, the preparation of tablet (can refer to existing conventional method. For example, according to the Guanxinkang tablet that the extraction process that provides in the drug standard WS3-B-2759-97 issued by the ministry is prepared) desirable Radix Glycyrrhizae is chopped, add water Decoct twice, decoct for 2 hours for the first time, decoct for 1 hour for the second time, filter, combine the filtrate, concentrate to an appropriate amount, add ethanol to make the alcohol content reach 65-70% (volume ratio concentration), let stand , filtered, the filtrate was concentrated to a thick paste, an appropriate amount of auxiliary materials was added, granulated by conventional methods, dried, compressed into tablets, and coated to obtain tablets.

[0075] 2. Take 20 tablets above, remove the sugar coating or film coating, grind the plain tablets finely, weigh about 1.0g of the powder, weigh it ac...

Embodiment 3

[0078] Embodiment 3 Application of detection method of the present invention

[0079] 1. Preparation of tablets: Take the leaves of Glycyrrhiza radicis, chop them, add ethanol to make the alcohol content reach 65-70%, reflux extraction twice, the first time for 2 hours, the second time for 1 hour, filter, combine the filtrates, Concentrate the filtrate to a thick paste (density: 1.3-1.4, measured at 80°C), add appropriate amount of excipients, conventionally granulate, dry, compress into tablets, and coat.

[0080] 2. Take 20 of the above-mentioned tablets, remove the sugar coating or film coating, grind the plain tablets finely, weigh about 1.0 g of the powder, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50 mL of 80% ethanol accurately, and weigh it. Ultrasonic (power 200w, frequency 40kHz) extraction 0.5 ~ 1h, let cool, and then weighed, with 80% ethanol to make up for the lost weight, shake well, filter, the filtrate is concentrated under reduced pressu...

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Abstract

The present invention discloses a detection method for a flavone component in clematis filamentosa dunn, and an application thereof. According to the present invention, a kaempferide-containing methanol solution is adopted as a reference substance solution; a high performance liquid chromatography method is adopted to carry out qualitative and / or quantitative analysis for the flavone component in a prepared clematis filamentosa dunn solution requiring detection. The method of the present invention can be applicable for detections of the flavone component in extracts, preparations, decoction pieces or medicinal materials, and no interference is generated to the negative result, wherein the extracts contain the clematis filamentosa dunn component, and the extracts are prepared by the same or the similar extraction methods. In addition, the method of the present invention has strong specificity and good reproducibility, such that the uniform, stable, effective and controllable preparation assortment of the clematis filamentosa dunn can be effectively ensured, and the process normalization, the quality stability and the quality uniformity are ensured.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a detection technology of flavonoid components in traditional Chinese medicines, decoction pieces, extracts and preparations, in particular to a detection method and application of flavonoid components in Ganmotong medicinal material extract. Background technique [0002] Clematis filamentosa Dunn (Clematis filamentosa Dunn), commonly known as cobra medicine, is a plant of the genus Clematis in the family Ranunculaceae. It is mainly distributed in Guangdong, Guangxi, Hainan, Yunnan and other places. There are relatively rich wild resources in Ruyuan mountainous area in northern Guangdong. Vascular diseases (coronary heart disease, hypertension) have a significant effect. Flavonoids in Ganmutong are one of its active ingredients. [0003] "Guanxinkang Tablets" produced with Ganmutong as a raw material has outstanding clinical effects in the treatment of coronary hear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 邓亚利
Owner SOUTH CHINA AGRI UNIV
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