Cdc45l peptides and vaccines including the same
A HLA-A24, antigen technology, applied in the field of drugs for the treatment and prevention of tumors, can solve the problem that the usefulness of CDC45L has not been fully investigated
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[0385] Materials and methods
[0386] cDNA microarray analysis
[0387] Gene expression profiles were generated using cDNA microarray analysis as previously described (Nakamura T et al., Oncogene 2004; 23:2385-400, Taniwaki M et al., Int J Oncol 2006; 29:567-75). Raw data for microarray analysis are available upon request from Prof. Y. Nakamura (The University of Tokyo, Graduate School of Medicine). Tissue samples from lung cancer and adjacent noncancerous normal lung tissue were obtained from surgical specimens, and all patients provided informed consent to participate in the study.
[0388] mouse
[0389] Six-week-old female non-obese diabetic (NOD) / severe combined immunodeficiency (SCID) mice were purchased from Charles River Laboratories Japan. The mice were bred at the Animal Resources and Development Center of Kumamoto University, and their operation complied with the animal care guidelines of Kumamoto University.
[0390] Cell lines and HLA expression
[0391] CD...
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