Method for detecting pig fat deposition by using phylaxin expression level

A pig fat and resistin technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as the inability to continuously and dynamically monitor pig fat deposition.

Inactive Publication Date: 2012-06-06
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing methods for detecting fat deposition in pigs are mainly to detect the backfat thickness of pigs, which needs to be completed after slaughtering, and it is impossible to continuously and dynamically monitor the fat deposition of pigs

Method used

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  • Method for detecting pig fat deposition by using phylaxin expression level
  • Method for detecting pig fat deposition by using phylaxin expression level
  • Method for detecting pig fat deposition by using phylaxin expression level

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Embodiment 1

[0024] The applicant provides a method for detecting fat deposition in pigs, the steps of which are as follows:

[0025] (1) extracting total RNA from the tested biological material, and obtaining its cDNA sample by reverse transcription;

[0026] (2) Perform PCR amplification on the cDNA sample described in step (1) with the primer pair shown in SEQ ID NO: 2 and 3 in the sequence table to obtain the gene fragment shown in SEQ ID NO: 1 in the sequence table;

[0027] (3) Using the primer pair shown in SEQ ID NO: 5 and 6 in the sequence table, perform PCR amplification on the housekeeping gene G3PDH to obtain the gene fragment shown in SEQ ID NO: 4 in the sequence table;

[0028] (4) Carry out agarose gel electrophoresis to the PCR product that step (2) and step (3) obtain, compare its optical density value in the gel imaging system;

[0029] Wherein the PCR amplification step described in step (2) is as follows:

[0030] 1) Amplification reaction system: In 50ul PCR reaction...

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Abstract

The invention relates to a method for detecting pig fat deposition by using phylaxin expression level, which comprises the following steps: 1) extracting total DNA from detected pig tissue, carrying out inverse transcription to obtain a cDNA sample; 2) carrying out PCR amplification on the cDNA sample from the step 1) by primer pair shown in SEQ ID NO:2 and 3 to obtain a gene fragment shown in SEQ ID NO:1; 3) carrying out PCR amplification on the housekeeping gene by primer pair shown in SEQ ID NO:5 and 6 to obtain a gene fragment shown in SEQ ID NO:4; 4) carrying out agarose gel electrophoresis on the PCR products from the step 2) and step 3), comparing the optical density value in a gel imaging system. According to the invention, the fat pigs and lean type pigs with 1 month age, 3 months age, 5 months age and 7 months age can be taken as detection objects, the expression level of fat cytokine phylaxin (resistin) can be detected, the protein level of resistin is taken for reflecting the deposition condition of the pig fat. The method of the invention has the advantages of rapidity, convenience, accuracy and no influence on normal growth of pigs, and is suitable for dynamically monitoring the fat deposition condition of pigs in the culture process.

Description

technical field [0001] The invention relates to a method for detecting fat deposition in pigs. Specifically, it relates to a method for dynamically monitoring fat deposition in pigs by using an adipocyte factor resistin. The method uses the expression level of resistin to detect fat deposition in pigs, so as to grasp the fat deposition situation of pigs at any time during the breeding process. Background technique [0002] The most important and widely known function of adipose tissue is to store energy in the form of triglycerides in times of energy surplus and to mobilize this energy in states of starvation. Adipose tissue has long been considered a passive, inactive tissue. However, discoveries in the past two decades have proved that adipose tissue is not only an energy storage organ, but also a major endocrine organ. It can secrete many factors, including leptin (leptin), tumor necrosis factor alpha (TNFa), adiponectin (adipnectin) and resistin (resistin), etc. play ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 杨在清周磊陈小冬雷霆
Owner HUAZHONG AGRI UNIV
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