Method for detecting pig fat deposition by using phylaxin expression level
A pig fat and resistin technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as the inability to continuously and dynamically monitor pig fat deposition.
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[0024] The applicant provides a method for detecting fat deposition in pigs, the steps of which are as follows:
[0025] (1) extracting total RNA from the tested biological material, and obtaining its cDNA sample by reverse transcription;
[0026] (2) Perform PCR amplification on the cDNA sample described in step (1) with the primer pair shown in SEQ ID NO: 2 and 3 in the sequence table to obtain the gene fragment shown in SEQ ID NO: 1 in the sequence table;
[0027] (3) Using the primer pair shown in SEQ ID NO: 5 and 6 in the sequence table, perform PCR amplification on the housekeeping gene G3PDH to obtain the gene fragment shown in SEQ ID NO: 4 in the sequence table;
[0028] (4) Carry out agarose gel electrophoresis to the PCR product that step (2) and step (3) obtain, compare its optical density value in the gel imaging system;
[0029] Wherein the PCR amplification step described in step (2) is as follows:
[0030] 1) Amplification reaction system: In 50ul PCR reaction...
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