Method for preparing ABPS

A polysaccharide and Achyranthes technology are applied in the field of preparation of plant polysaccharides, can solve the problems of low purity of Achyranthes polysaccharide, cannot meet preparation requirements, etc., and achieve the effects of low equipment and environmental requirements, low cost, and favorable promotion.

Inactive Publication Date: 2012-08-01
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of the polysaccharide obtained from Achyranthes bidentata is not high enough to meet the current increasing requirements for the preparation of preparations. In view of this, it is particularly necessary to develop a new method for preparing polysaccharides from the crude drug Achyranthes bidentata.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Cut Achyranthes bidentata into slices, and soak it in distilled water at a mass ratio of 1:6 with water for 50 hours;

[0024] (2) Centrifuge the soaking solution in (1) at 3000rpm for 16min to obtain the supernatant containing Achyranthes bidentata polysaccharide;

[0025] (3) Add 95% ethanol of 3 times its volume to the supernatant of (2), and stir for 25 minutes;

[0026] (4) centrifuge the mixed solution of (3) at 3000 rpm for 16 minutes, take the precipitate and dilute it with distilled water to a solution with a specific gravity of 1.10 to 1.15;

[0027] (5) Select a chromatographic column with an inner diameter and a height ratio of 1:30; add SephadexG-150 dextran gel to the column, and the amount added is 75% of the column volume;

[0028] (6) The solution of gel filtration chromatography step (4), the eluent is distilled water, the flow rate is 0.4ml / min, and the wavelength is 280nm to detect the collected liquid, and the eluent is selected to collect the ...

Embodiment 2

[0031] (1) Crush Achyranthes bidentata until the particle diameter is 400-500 μm, and soak it in distilled water at a mass ratio of 1:7 with water for 60 hours;

[0032] (2) Centrifuge the soaking solution in (1) at 4000rpm for 15min to obtain the supernatant containing Achyranthes bidentata polysaccharide;

[0033] (3) Add 90% ethanol of 4 times its volume to the supernatant of (2), and stir for 35 minutes;

[0034] (4) Centrifuge the mixed solution of (3) at 4000 rpm for 15 minutes, take the precipitate and dilute it with distilled water to a solution with a specific gravity of 1.11 to 1.15;

[0035] (5) Select a chromatographic column with an inner diameter and a height ratio of 1:40; add SephadexG-200 dextran gel to the column, and the amount added is 85% of the column volume;

[0036] (6) The solution of gel filtration chromatography step (4), the eluent is distilled water, the flow rate is 0.4ml / min, and the wavelength is 280nm to detect the collected liquid, and the el...

Embodiment 3

[0039] (1) Crush Achyranthes bidentata until the particle diameter is 300-400 μm, and soak it in distilled water at a mass ratio of 1:8 with water for 70 hours;

[0040] (2) Centrifuge the soaking solution in (1) at 5000rpm for 13min to obtain the supernatant containing Achyranthes bidentata polysaccharide;

[0041] (3) Add 95% ethanol of 3 times its volume to the supernatant of (2), and stir for 30 min;

[0042] (4) Centrifuge the mixed solution of (3) at 5000 rpm for 13 minutes, take the precipitate and dilute it with distilled water to a solution with a specific gravity of 1.05 to 1.10;

[0043] (5) Select a chromatographic column with an inner diameter and a height ratio of 1:50; add SephadexG-150 dextran gel to the column, and the amount added is 80% of the column volume;

[0044] (6) The solution of gel filtration chromatography step (4), the eluent is distilled water, the flow rate is 0.5ml / min, and the wavelength is 280nm to detect the collected liquid, and the eluent...

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Abstract

The invention discloses a method for preparing ABPS (achyranthes bidentata polysaccharides). Dipping achyranthes into distilled water after the achyranthes is cut into sheets or crushed, obtaining supernatant fluid containing ABPS through centrifugation, then adding ethanol for stirring and centrifugation, centrifuging and collecting precipitates and diluting by using distilled water, carrying out gel filtration chromatography, selecting absorption peak eluate for collection, and carrying out vacuum freezing and drying, so as to obtain the ABPS. The purity of the ABPS prepared by using the method provided by the invention can reach more than 95 percent, and the method has low requirements on equipment and environment, is simple convenient, flexible and practical, has a low cost and is favorable for popularization, development and application.

Description

technical field [0001] The invention relates to a method for preparing plant polysaccharides, in particular to a method for preparing polysaccharides from the crude drug Achyranthes bidentata. Background technique [0002] Achyranthes bidentata (scientific name: Achyranthes bidentata) is a plant of the genus Achyranthes in the family Amaranthaceae. Achyranthes bidentata, as a traditional Chinese medicinal material in my country, has recorded its unique clinical application value from Li Shizhen's "Compendium of Materia Medica" in the Ming Dynasty to Ye Tianshi's "Compendium of Materia Medica" in the Qing Dynasty, as well as the "Dictionary of Chinese Medicine" in modern medicine: nourishing the liver and kidney, Strengthen muscles and bones, treat soreness and numbness in the waist and knees, dizziness in the liver and intestines, dissipate blood stasis, reduce carbuncle, etc. [0003] Achyranthes bidentata polysaccharide (ABP) is extracted from the root of Achyranthes biden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张尚立张恒
Owner SHANDONG UNIV
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