Process of preparing high purity astragalus polysaccharide

An astragalus polysaccharide and high-purity technology is applied in the field of preparation of plant polysaccharides, and can solve the problems of inability to meet preparation requirements, roughness and low purity of astragalus polysaccharide products, low requirements for equipment and environment, favorable for promotion, and difficult separation. Effect

Inactive Publication Date: 2007-08-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are also reports involving the extraction and separation method of Astragalus polysaccharides, the obtained Astragalus polysaccharide products are relatively rough and the purity is not high, which can no longer meet the increasing requirements for the preparation of preparations. The method of pure astragalus polysaccharide should be the top priority in the study of astragalus polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Grind Astragalus membranaceus until the particle diameter is 200-300 μm, immerse it in distilled water according to the mass ratio of 1:8, and carry out constant temperature microwave extraction at 65 ° C. Extraction conditions: microwave power 250W, time 13 minutes, Repeat 3 times;

[0027] (2) Centrifuge the extraction mixture described in (1) at 4000rpm for 15min, take the supernatant, decolorize it with activated carbon, and obtain the aqueous extract solution of astragalus polysaccharide;

[0028] (3) concentrating the astragalus polysaccharide aqueous extract solution to a specific gravity of 1.15 to 1.20;

[0029] (4) Add 85% ethanol to the concentrated astragalus polysaccharide aqueous extract solution in an amount 7 times the volume of the concentrated astragalus polysaccharide aqueous extract solution, and stir for 26 minutes;

[0030] (5) Centrifuge the mixed solution of (4) at 4000 rpm for 15 minutes, take the precipitate and dilute it with distilled wa...

Embodiment 2

[0035] (1) Grind Astragalus membranaceus until the particle diameter is 200-300 μm, immerse it in distilled water at a mass ratio of 1:6 with water, and carry out constant temperature microwave extraction at 75°C. Extraction conditions: microwave power 250W, time 11 minutes, Repeat 2 times;

[0036] (2) Centrifuge the extraction mixture described in (1) at 3000rpm for 16min, take the supernatant, and decolorize it with activated carbon to obtain the aqueous extract solution of astragalus polysaccharide;

[0037] (3) concentrating the astragalus polysaccharide aqueous extract solution to a specific gravity of 1.15 to 1.2;

[0038] (4) Add 70% ethanol to the concentrated astragalus polysaccharide aqueous extract solution in an amount 6 times the volume of the concentrated astragalus polysaccharide aqueous extract solution, and stir for 20 minutes;

[0039] (5) centrifuge the mixed solution of (4) at 3000 rpm for 16 minutes, take the precipitate and dilute it with distilled wate...

Embodiment 3

[0044] (1) Grind Astragalus membranaceus until the particle diameter is 200-300 μm, immerse it in distilled water at a mass ratio of 1:10 with water, and carry out constant temperature microwave extraction at 50°C. Extraction conditions: microwave power 250W, time 15 minutes, Repeat 3 times;

[0045] (2) Centrifuge the extraction mixture described in (1) at 5000rpm for 12min, take the supernatant, and decolorize it with activated carbon to obtain the aqueous extract solution of astragalus polysaccharide;

[0046] (3) concentrating the astragalus polysaccharide aqueous extract solution to a specific gravity of 1.2 to 1.25;

[0047] (4) Add 95% ethanol to the aqueous extract solution of astragalus polysaccharide in an amount of 6 to 8 times the volume of the concentrated astragalus polysaccharide aqueous extract solution, and stir for 36 minutes;

[0048] (5) Centrifuge the mixed solution of (4) at 5000 rpm for 12 minutes, take the precipitate and dilute it with distilled water...

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Abstract

The process of preparing high purity astragalus polysaccharide includes the following steps: crushing astragalus root and constant temperature microwave extracting in distilled water at 50-75 deg.c, centrifuging; taking supernatant and decolorizing with active carbon to obtain solution of astragalus polysaccharide; concentrating and adding alcohol via stirring; centrifuging and dissolving the precipitate in distilled water; filtering the gel and chromatography; detecting the collected liquid at 280 nm wavelength and collecting eluted liquid at the absorption peak; and vacuum freeze drying to obtain the astragalus polysaccharide product. The present invention has astragalus polysaccharide product purity over 90 %, relaxed preparing process, low cost and other advantages.

Description

technical field [0001] The invention relates to a method for preparing plant polysaccharides, in particular to a method for separating and preparing high-purity astragalus polysaccharides from traditional Chinese medicine astragalus. Background technique [0002] Radix Astragali (Radix Astragali), also known as Mianhuangqi, English MilkvetchRoot, is the root of the leguminous plant Astragalus membranaceus Bge.var.mongholicus (Bge.) Hsiao. Astragalus membranaceus has the functions of invigorating qi and consolidating the surface, detoxifying and discharging pus, diuretic, astringing sores and promoting granulation; it is often used clinically for qi deficiency and fatigue, chronic diarrhea and prolapse of the anus, spontaneous sweating, edema, prolapse of the genitals, chronic nephritis, proteinuria, diabetes, and chronic sores. Diseases such as non-healing. [0003] In recent decades, it has been found that polysaccharides extracted from plants have very important and speci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张尚立常宏文马剑峰
Owner SHANDONG UNIV
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