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Barcode preparation method used for individual animal identity identification and/or meat product tracing and application thereof

A technology of identification and barcode, applied in the field of molecular biology

Active Publication Date: 2012-09-19
江苏优源奶业产业研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Almost all common SNPs have only two alleles, but in an individual, the paired chromosomes may be different alleles

Method used

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  • Barcode preparation method used for individual animal identity identification and/or meat product tracing and application thereof
  • Barcode preparation method used for individual animal identity identification and/or meat product tracing and application thereof
  • Barcode preparation method used for individual animal identity identification and/or meat product tracing and application thereof

Examples

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Embodiment 1

[0023] Example 1 Production of individual barcodes for 96 pigs in Liuhe Base Pig Farm, Jiangsu Academy of Agricultural Sciences

[0024] 1.1 Genomic DNA template preparation

[0025] Select 5-month-old pigs (a total of 96 pigs, born to 7 breeding boars and 12 breeding sows. Among them, 1-9, 10-17, 18-24, 25-32, 33-37 . Insert 8.8 μl of DNA MiniExtract (Nanjing Runbang Biotechnology Co., Ltd.) into a PCR tube (the hair follicle end faces down and immerse in the MiniExtract), put the PCR tube in a PCR machine at 95°C for 5min, and at 16°C for 5min, add 1.2μl proteinase K ( 10mg / ml), at 55°C for 2hr or above, at 95°C for 10min, at 16°C for 1min, centrifuge the supernatant as a PCR template.

[0026] 1.2PCR amplification

[0027] Primer design: Primer pairs 1-7 were designed according to the genes with accession numbers AF327369, AF034974, AF473820, X68247, AJ251197, AF038553, and M29939 in GenBank, see Table 1 for details.

[0028] Reaction system (20μl): double distilled water...

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Abstract

The invention belongs to the field of molecular biology, and discloses a barcode preparation method used for individual animal identity identification and / or meat product tracing and application thereof. The method comprises the following steps of selecting single nucleotide polymorphism (SNP) sites with high heterozygosity on an animal to be identified or a meat product genome DNA to be traced and combining the SNP sites into an SNP barcode, and then using ten Arabic numbers 0-9 to randomly and uniquely replace the ten SNP gene types: A / A, T / T, G / G, C / C, A / T, A / G, A / C, T / G, T / C and G / C in the SNP barcode, forming corresponding numerical barcodes, and realizing correspondence of individual animal identities and barcodes one by one. According to the method provided by the invention, as a polymerase chain reaction (PCR) primer is designed, the SNP sites with high heterozygosity can be obtained, and the sites can be used for preparing the SNP barcode and / or corresponding numerical barcode used for identifying the individual animal identity and / or tracing the meat product, thereby realizing individual animal identity identification and meat product tracing.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a barcode compilation method for animal individual identification and / or traceability of meat products and its application. Background technique [0002] Pork is the most important meat product consumed by Chinese residents, and my country is also the country with the largest production and consumption of pork. Food safety has frequently occurred in recent years and has become the focus of public opinion. Establish a reliable traceability system for pork products, realize two-way traceability from the pig farm to the dining table, curb the production of pork with problems such as veterinary drug residues, clenbuterol, and heavy metal abuse, and cut off infectious diseases, especially zoonotic infectious diseases And processing, the cultivation of market consumer confidence, the healthy development of the pig industry, etc., play a vital role. [0003] There are many technical met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K19/06
Inventor 邢光东胡肄农赵庆顺顾洪如王晓晓朱振坤施振旦
Owner 江苏优源奶业产业研究院有限公司
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