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Four cassane type diterpenoid compounds with antineoplastic activities

A technology of anti-tumor activity and compounds, applied in anti-tumor drugs, steroids, organic chemistry, etc., can solve the problems of no patents or literature reports

Inactive Publication Date: 2013-01-09
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The compounds Minaxin A (I), Neocaesalpin AA (II), Neocaesalpin AB (III), and Neocaesalpin AD (IV) involved in the present invention are all new compounds, and their antitumor activity is discovered for the first time. So far, the above research results have not yet been published. Patent or literature report

Method used

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  • Four cassane type diterpenoid compounds with antineoplastic activities
  • Four cassane type diterpenoid compounds with antineoplastic activities
  • Four cassane type diterpenoid compounds with antineoplastic activities

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Cytotoxicity test:

[0041] The activity of each compound was screened by MTT colorimetry. Select human cervical cancer cell Hela, human colon cancer cell HCT-8 culture medium diluted, with 6 × 10 4 Inoculate in a 96-well plate at a density of / ml, 100 μl per well. After 24 hours of normal culture in the incubator, add the drug. The final concentrations of the drugs were 2.5 μg / ml (group 1), 5 μg / ml (group 2), 10 μg / ml (group 3), 20 μg / ml (group 4), and 40 μg / ml (group 5). A total of 5 concentrations were set up, with 3 replicate wells for each concentration, and a blank group was set up. After culturing for 48 hours, add 10 μl of MTT to each well for staining. After continuing to cultivate for four hours, discard the original culture solution, add 100 μl of DMSO to each well, shake it on a shaker at a low speed for 10 minutes to fully dissolve the crystals, and detect the optical density value at a wavelength of 570nm by an enzyme-linked immunosorbent detector. The ...

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Abstract

The invention relates to four novel cassane type diterpenoid compounds which are obtained by separating from semen caesalpiniae, nanely seed of caesalpinia minax hance of caesalpinia genus of Leguminosae, through a phytochemical extraction separation way. The invention also relates to cytotoxic effects of the compounds on cervical cancer and colon cancer, and applications of the compounds on clinical prevention and treatment of tumors.

Description

Technical field: [0001] The present invention relates to extracting and separating 4 new Kazanane-type diterpenoids and the like from the seed of Caesalpinia minax Hance through phytochemical extraction and separation means. The cytotoxic effect of the compound on cervical cancer and colon cancer, and its application in the clinical prevention and treatment of tumors. Background technique: [0002] The bitter stone lotus is the seed of Caesalpinia minax Hance in the genus Fabaceae. Commonly known as Nanshele and Dragon's Claw, it is a low shrub that grows in mountains, valleys, river valleys or roadside bushes at an altitude of 150-680m. In my country, it is mainly distributed in Guangxi and Yunnan in Southwest and South China. , Guizhou, Guangxi, Guangdong, Hainan and other places. Shilian bitterness is cool in nature, bitter in taste, has the effects of clearing away heat and dampness, promoting blood circulation and dispelling blood stasis, and relieving pain. It is a com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00C07D307/77A61P35/00
Inventor 许旭东马国需曹丽袁经权马丽焱吴海峰吴丽珍郑庆霞钟明亮杨峻山
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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