Shuttle expression vector with broad hosts

Abstract

The invention discloses a shuttle expression vector with broad hosts and provides a plasmid. The plasmid comprises the following components: a replication origin oriV, a gene for encoding replication protein Rep, a conjugation origin oriT, a gene for encoding conjugated protein Mob, an antibiotic selective marker gene and a foreign gene expression cassette, wherein the foreign gene expression cassette sequentially comprises a promoter, a multiple cloning endonuclease site in which a foreign gene is inserted, and a transcription termination sequence from upstream to downstream. The plasmid has a broad host ability, can be stably replicated in hosts and has high transconjugative frequency. The shuttle expression vector has great value for the genetic transformation of Acidithiobacillus caldus.

Description

technical field

[0001] The invention relates to a shuttle expression vector with a wide range of hosts. Background technique

[0002] Biometallurgy is one of the very active disciplines in the field of metallurgy in the past two decades, and has achieved industrial applications in the extraction of copper, gold, uranium and other metals. Biometallurgy is a technology that oxidizes, reduces or complexes metal minerals through the use of microorganisms, allowing metal ions to enter the solution for further separation, enrichment, and purification to extract metals. Compared with conventional physical and chemical dressing and smelting methods, biometallurgy has outstanding advantages such as simple process, low cost, low energy consumption, and little environmental pollution. , Heap leaching and in-situ leaching of refractory ores. In today's situation where there is a large demand for resources, the shortage of easy-to-develop resources, and the continuous improvement of en...

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