Preparation method of feed protein peptide without probiotic bacteria activity resistance
A feed protein and living technology, applied in the field of feed protein peptide preparation, can solve the problems of not being able to meet the growth requirements of various flora
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[0025] 1) Pick a ring of bacteria from the Saccharomyces cerevisiae, Geotrichum candidum, Candida adenidae, and Candida tropicalis from the Saccharomyces cerevisiae species preserved on the slant, inoculate them into 0.5 L wort seed medium, and inoculate them at 28°C Temperature, 120rpm, shaker culture for 48 hours, so that the number of viable bacteria reached more than 106 / ml, equal volumes of mixed seed culture solution of the four strains, to obtain 2L yeast compound seed solution.
[0026] 2) Pick a ring of bacteria from the original species of Aspergillus oryzae and Aspergillus niger that were preserved on the slant, inoculate them into 1 L of Chase seed medium, and culture them on a shaker at 28°C and 120rpm for 72 hours , stop the cultivation, make the number of viable bacteria reach more than 106 / ml, mix the seed culture solution of the two strains in equal volumes, and obtain 2L mold compound seed solution.
[0027] 3) Mix 2L yeast compound seed liquid and 2L mold co...
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