Cordyceps specimen preservation solution

A preservation solution and specimen technology, applied in the preservation of plants, botanical equipment and methods, applications, etc., can solve problems such as easy moths or mildew, obvious changes in the shape and color of spores, unfavorable specimen preservation or follow-up research, etc. , to achieve the effect of simple and practical operation

Inactive Publication Date: 2013-04-10
YUNNAN UNIV CHINESE HERBAL MEDICINE BIOLOGICAL RESOURCE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are two main ways to preserve Cordyceps specimens. One is the traditional dry specimen preservation method. After the Cordyceps is dried, the sclerotium changes slightly, and the shape and color of the stroma change significantly, and it is easy to be eaten by insects or mildew, which is not conducive to Specimen preservation or follow-up research; another method is to use preservation solution for preservation, mainly using formalin into the solution to preserve, the shape of Cordyceps basically does not change, but the color of Cordyceps sclerotia and sub-seats is easy to fade, especially some unstable colors such as light yellow, orange, orange-red, red, and green

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Example 1 Preparation of Cordyceps militaris specimens

[0019] The color of Cordyceps militaris (L.exFr.) Link is orange-yellow to orange-red, and the color of the sclerotia is dark brown.

[0020] 1 Pretreatment of Cordyceps specimens

[0021] Firstly, remove dirt and other sundries on the surface of Cordyceps militaris, and wash the Cordyceps with clean water; secondly, remove the caps wrapped around the sclerotia of Cordyceps, and wash them with distilled water;

[0022] 2 fixation of Cordyceps specimens

[0023] Use transparent nylon thread to fix the Cordyceps militaris specimen on a transparent glass plate of suitable size.

[0024] 3 preparation of Cordyceps soaking solution

[0025] Formula: 1.0% acetic acid, 1.5% formaldehyde, 0.5% sulfurous acid, 5.0% glycerin, 0.5% ethanol. After the solution is prepared, pour it into a transparent glass jar.

[0026] 4 Put the fixed Cordyceps specimens into the jar, cover the lid, seal the mouth of the bottle with para...

example 2

[0027] The making of example two Cordyceps sinensis specimen

[0028] The part of Cordyceps sinensis (Berk.) Sacc. near the sclerotium is white to white-gray, and the surface color of the sclerotia is light yellow to dark yellow.

[0029] 1 Pretreatment of Cordyceps specimens

[0030] Firstly remove the soil and other debris on the surface of Cordyceps sinensis, and wash the Cordyceps sinensis with clean water; secondly, remove the fungus wrapping the Cordyceps sclerotia, and wash it with distilled water;

[0031] 2 fixation of Cordyceps specimens

[0032] Use transparent nylon thread to fix the Cordyceps sinensis specimen on a transparent glass plate of suitable size.

[0033] 3 preparation of Cordyceps soaking solution

[0034] Formula: 1% acetic acid, 1.5% formaldehyde, 0.2% sulfurous acid, 5% glycerin, 0.5% ethanol. After the solution is prepared, pour it into a transparent glass jar.

[0035] 4 Put the fixed Cordyceps specimens into the jar, cover the lid, seal the m...

example 3

[0036] Examples of preparation of Cordyceps specimens in Sanmeitan

[0037] The sclerotia and subunits of Cordyceps indigotica Kobayasi et Shimizu are light green.

[0038] 1 Pretreatment of Cordyceps specimens

[0039] Firstly, remove dirt and other sundries on the surface of Cordyceps Meitan, and wash the Cordyceps with clean water; secondly, remove the caps wrapped around the sclerotia of Cordyceps, and wash with distilled water; then soak in saturated copper sulfate solution for 12 hours, and then wash with distilled water.

[0040] 2 fixation of Cordyceps specimens

[0041] Use a transparent nylon thread to fix the Cordyceps meitan specimen on a transparent glass plate of suitable size.

[0042] 3 preparation of Cordyceps soaking solution

[0043] Formula: 1% acetic acid, 1.5% formaldehyde, 0.5% copper acetate, 3% glycerin, 0.5% ethanol. After the solution is prepared, pour it into a transparent glass jar.

[0044] 4 Put the fixed Cordyceps specimens into the jar, co...

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PUM

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Abstract

The present invention relates to a preparation method of a cordyceps specimen preservation solution. The formula includes 1.0% of acetic acid, 0.5% of copper acetate (the composition is added selectively according to the color of cordyceps), 1.5% of formaldehyde, 0.2-0.5% of sulphurous acid (the composition is added selectively according to the color of cordyceps), 2.0-5.0% of glycerol, and 0.5% of ethanol. The solution can not only maintain shapes of cordyceps, but also keep the color, so that the cordyceps specimens are better preserved.

Description

technical field [0001] The invention relates to a preservation liquid formula suitable for different kinds of cordyceps. Background technique [0002] Cordyceps Cordyceps (Fr.) Link belongs to Fungi Kingdom (Fungi), Ascomycota Ascomycota, Ascomycetes Ascomycetes, Sordariomycetidae, Hypocreales Hypocreales, Clavicipitaceae Clavicipitaceae (Kirk et al.2001) . According to the statistics of Yoshio Kobayashi and Daisei Shimizu (1983), there are more than 350 kinds of Cordyceps reported in the world. Subsequently, the species names listed in the International Fungi List Database in 2003 have reached more than 485 species, and with the addition of new species published in recent years at home and abroad, there have been more than 500 species of Cordyceps reported in the world. Excluding synonyms, illegal abandoned names and species moved to other genera, there should be about 400 species of Cordyceps. [0003] After the Cordyceps mycelium invades the insect host, most of them f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 虞泓曾文波杨俊媛陈自宏
Owner YUNNAN UNIV CHINESE HERBAL MEDICINE BIOLOGICAL RESOURCE INST
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