Method for exogenous gene expression optimization in salmonella
A Salmonella and exogenous gene technology, applied in the field of optimizing the expression of exogenous genes in Salmonella, to achieve the effect of increasing the expression amount and optimizing the expression
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[0023] Experiment 1: Optimal expression of phage E gene in Salmonella
[0024] 1. First design and synthesize phage E gene-specific primers
[0025] E 1S: ATGGTACGCTGGACTTTGTGGGA 3'
[0026] E 1A: TCACTCCTTCCGCACGTAATTTTTG 3'
[0027] 2. The E gene was obtained by PCR amplification and sequenced, and its sequence was: ATGGT A CGCTGGAC T TTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAG T TTATTGCTGCCGTC A TTGCT T ATTATGTTCATCCCGTC A AC A TTCAAACG G CC T GTCTC A TC A TGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAA G CCGCTGAATTG T TCGCGTTTACC T TGCGTGTA C GCGCAGGAAAC A CTGACGTTCTTACTGACGCAGAAGAAAAC G TGCGTCAAAAATTA C GTGCG G AAA G GAGTGA.
[0028] 3. Then according to the Salmonella codon usage frequency table, i.e. Table 1 and the amino acid codon table, i.e. Table 2, point mutation is carried out to the E gene, so that its low frequency usage codon becomes a high frequency usage codon, and the E gene mutation The sequence is: ATGGT G CGCTGGAC G TTGTGGGATACCCT...
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