Reagent kit for auxiliary diagnosis of lung adenocarcinoma paitent

A technology for auxiliary diagnosis and lung adenocarcinoma, applied in biological testing, material testing products, measuring devices, etc., can solve problems such as low diagnostic efficiency and failure to meet clinical needs, and achieve high sensitivity and specificity.

Active Publication Date: 2013-06-19
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Although the above protein markers have been used in the auxiliary diagnosis of lung cancer, their diagnostic efficiency is low and cannot meet the clinical needs

Method used

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  • Reagent kit for auxiliary diagnosis of lung adenocarcinoma paitent
  • Reagent kit for auxiliary diagnosis of lung adenocarcinoma paitent
  • Reagent kit for auxiliary diagnosis of lung adenocarcinoma paitent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Establishment of the method of the present invention

[0033] 1. Model establishment

[0034] There were 473 plasma samples from patients with lung adenocarcinoma and 479 plasma samples from healthy people (normal controls). They were taken from lung cancer patients who were diagnosed pathologically by the Department of Thoracic Surgery, Cancer Hospital of the Chinese Academy of Medical Sciences and normal people who participated in the cancer screening. This experiment was approved by the ethics committee, and the subjects were informed and signed an informed consent form. Among patients with lung adenocarcinoma, there were 239 males and 234 females, ranging from 26 to 87 years old, with an average age of 60.50 years; in the normal population, 240 males and 239 females, ranging from 41 to 70 years old, with an average age Is 57.39 years old. The plasma samples were obtained before surgery, and the patient had not received chemotherapy, radiotherapy, or inter...

Embodiment 2

[0045] Example 2. Verification of the method of the invention

[0046] The patients used in this example are 150 clinically diagnosed lung adenocarcinoma patients (peripheral venous blood was obtained before surgery, and the patients had not received chemotherapy, radiotherapy, intervention, etc.) and 50 healthy people (non-lung adenocarcinoma) Patients), all volunteers with informed consent.

[0047] 1. Take 5ml of peripheral venous blood from the patient under fasting condition, leave it at room temperature for 1h, then centrifuge at 3000r / min for 15min, take the supernatant, divide it into two 1.5mL EP tubes, and store at -80℃ in the refrigerator.

[0048] 2. Dissolve the sample of step 1 at room temperature for 3 hours, and then centrifuge at 3000 r / min for 5 minutes.

[0049] 3. The concentration of protein marker IDH1 (unit: U / L), the concentration of protein marker CA125 (unit: U / mL), and the concentration of protein marker CYFRA211 (unit: ng / mL) and the concentration of prot...

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Abstract

The invention discloses a reagent kit for an auxiliary diagnosis of a lung adenocarcinoma patient. The reagent kit comprises a first product, a second product, a third product and a carrier. The first product is used for detecting protein markers IDH1, the second product is used for detecting protein markers CA125, the third product is used for detecting protein markers CYFRA21-1, and the carrier records the following functional expression: x1 represents the concentration of the IDH1, x2 represents the concentration of the CA125, and x3 represents the concentration of the CYFRA21-1. By means of the reagent kit for the auxiliary diagnosis of the lung adenocarcinoma patient, the lung adenocarcinoma patient is diagnosed in an auxiliary mode according to corresponding diagnostic criteria. Furthermore, the reagent kit for the auxiliary diagnosis of the lung adenocarcinoma patient has the advantages of high sensitivity and strong specificity. In addition, the reliability of the diagnosis is far higher than that of the diagnosis of adopting each individual protein marker, so that the reagent kit for the auxiliary diagnosis of the lung adenocarcinoma patient has a significant value and an application prospect for the diagnosis and treatment of lung adenocarcinoma.

Description

Technical field [0001] The invention relates to a kit for assisting diagnosis of patients with lung adenocarcinoma. Background technique [0002] Lung cancer is a major disease that seriously threatens human life and health. In 2011, the World Health Organization (WHO) International Agency for Research on Cancer (IARC) released the 2008 global statistical data, showing that lung cancer is a malignant tumor with the highest morbidity and mortality. Every year, there are 1.608 million new cases and 1.377 million deaths worldwide, accounting for 12.7% and 18.2% of all malignant tumors. According to statistics released by the American Cancer Society, it is estimated that in 2012, the number of new lung cancer cases in the United States will reach 226,000, and the number of deaths due to lung cancer will reach 160,000. In my country, the results of the third national retrospective sample survey of death causes hosted by the Ministry of Health show that lung cancer is the leading cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
Inventor 赫捷谭锋维孙楠陈照丽
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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