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Kit for detecting goat pox virus

A technology of goat pox virus and kit, applied in the direction of microbe-based method, microbe measurement/inspection, microbiology, etc., can solve problems such as inability to distinguish goat pox virus genus, inability to distinguish viruses of the same genus and different species, etc. , to achieve the effect of high sensitivity, high detection sensitivity and simple detection operation

Active Publication Date: 2014-09-17
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the above-mentioned published methods or kits cannot distinguish viruses of the same genus and different species, that is to say, it is impossible to distinguish the genus of goatpox virus from the book of goat pox virus

Method used

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  • Kit for detecting goat pox virus
  • Kit for detecting goat pox virus
  • Kit for detecting goat pox virus

Examples

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Embodiment Construction

[0016] The present invention will be explained in detail below in conjunction with the examples.

[0017] 1. Preparation of Sequences

[0018] The amplification primers of the present invention are designed using relevant primer design software to design the reversed repeat ends (within 1-2000bp) of the sheeppox virus genome, and then select the primers with higher scores from the hundreds of pairs of primers obtained, and then insert the designed The sequence was sent to Nanjing GenScript Bioengineering Co., Ltd. for 5' end modification and synthesis. The sequences that were finalized were:

[0019] SEQ №1: ACCAAAACAAATAATCAGAGATG, named GTPV F3 in the present invention;

[0020] SEQ №2: CCTAATCCATTTAAGACACTACG, named GTPV B3 in the present invention;

[0021] SEQ №3: AAGATGTCTTCCGGTAACTATGTCT-GCGGTTGTGATATCGTCA, named GTPV FIP in the present invention

[0022] SEQ No. 4: CCGAACTTGTTATTTCTTGTGCTT-CACAATAAGGAACCACCTAGA, named GTPV BIP in the present invention.

[0023] 2....

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Abstract

The invention discloses a kit for detecting goat pox virus. Include at least four primers of SEQ No. 1, SEQ No. 2, SEQ No. 3 and SEQ No. 4 in the kit of the present invention, also have dNTP, Tris-HC, KCl, (NH4) 2SO4, MgSO4, Tween20 and Bst in the kit enzyme, and template DNA. The primer of the invention has specificity, and cannot amplify the nucleic acid of sheep pox virus under the same conditions, and meanwhile, the detection sensitivity of the invention is high, and meanwhile, the detection operation is relatively simple and fast.

Description

technical field [0001] The invention relates to a veterinary detection kit, specifically a kit for detecting goat pox virus. Background technique [0002] Capripox (CP) includes sheep pox, goat pox and bovine skin pimple disease, the first two of which are caused by sheep pox virus (Sheeppox virus, SPPV) and goat pox virus (Goatpox virus, GTPV) respectively. contact infectious diseases. The body temperature of sick animals rises, papules or nodules, blisters, visceral lesions and even death appear on the whole body, which has caused serious economic losses to the sheep industry in the world. The World Organization for Animal Health (OIE) stipulates it as an animal disease that must be reported by law, and my country lists it as a first-class animal infectious disease. The differential diagnosis of sheep pox and goat pox has been a research hotspot. [0003] The differential diagnosis of sheep pox and goat pox has been a research hotspot. Studies have shown that the homo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 张强赵志荀范斌吴国华颜新敏李健朱海霞芦晓立孙晓林刘发央
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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