Kit for detecting goat pox virus

A technology of goat pox virus and kit, applied in the direction of microbe-based method, microbe measurement/inspection, microbiology, etc., can solve problems such as inability to distinguish goat pox virus genus, inability to distinguish viruses of the same genus and different species, etc. , to achieve the effect of high sensitivity, high detection sensitivity and simple detection operation
CN103215385BActive Publication Date: 2014-09-17LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2014-09-17

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Abstract

The invention discloses a kit for detecting goat pox virus. Include at least four primers of SEQ No. 1, SEQ No. 2, SEQ No. 3 and SEQ No. 4 in the kit of the present invention, also have dNTP, Tris-HC, KCl, (NH4) 2SO4, MgSO4, Tween20 and Bst in the kit enzyme, and template DNA. The primer of the invention has specificity, and cannot amplify the nucleic acid of sheep pox virus under the same conditions, and meanwhile, the detection sensitivity of the invention is high, and meanwhile, the detection operation is relatively simple and fast.
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Description

technical field

[0001] The invention relates to a veterinary detection kit, specifically a kit for detecting goat pox virus. Background technique

[0002] Capripox (CP) includes sheep pox, goat pox and bovine skin pimple disease, the first two of which are caused by sheep pox virus (Sheeppox virus, SPPV) and goat pox virus (Goatpox virus, GTPV) respectively. contact infectious diseases. The body temperature of sick animals rises, papules or nodules, blisters, visceral lesions and even death appear on the whole body, which has caused serious economic losses to the sheep industry in the world. The World Organization for Animal Health (OIE) stipulates it as an animal disease that must be reported by law, and my country lists it as a first-class animal infectious disease. The differential diagnosis of sheep pox and goat pox has been a research hotspot.

[0003] The differential diagnosis of sheep pox and goat pox has been a research hotspot. Studies have shown that the homo...

Claims

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