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Method for establishing high performance liquid chromatography fingerprint spectrum of Exocarpium Juglandis Immaturum antioxidant active extract, its standard fingerprint spectrum and use

A technology of high performance liquid chromatography and antioxidant activity, which is applied in the field of establishment of high performance liquid chromatography fingerprints, can solve the problems of no standard fingerprints of Qinglongyi antioxidant extracts, achieve stable and reliable precision, reduce the possibility, good reproducibility

Inactive Publication Date: 2014-07-23
HEILONGJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Modern studies have found that Qinglongyi has cytotoxicity, antibacterial, anti-inflammatory, analgesic and significant anti-cancer activities, but its antioxidant activity has not been reported. , how to use scientific and reasonable methods to control its quality and closely related to its activity is a new idea and trend of quality control research methods at present, and it is very necessary to ensure the safety and effectiveness of clinical medication
Fingerprint, as a technique for overall quality control of traditional Chinese medicine, has been included in the 2010 edition of the Chinese Pharmacopoeia, and its advantages are more obvious than single-component or even multi-component qualitative and quantitative. There are currently researches on the fingerprint of Qinglongyi. According to a small number of literature reports, there is no method to establish the fingerprint of Qinglongyi antioxidant extract, so there is no standard fingerprint of Qinglongyi antioxidant extract

Method used

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  • Method for establishing high performance liquid chromatography fingerprint spectrum of Exocarpium Juglandis Immaturum antioxidant active extract, its standard fingerprint spectrum and use
  • Method for establishing high performance liquid chromatography fingerprint spectrum of Exocarpium Juglandis Immaturum antioxidant active extract, its standard fingerprint spectrum and use
  • Method for establishing high performance liquid chromatography fingerprint spectrum of Exocarpium Juglandis Immaturum antioxidant active extract, its standard fingerprint spectrum and use

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specific Embodiment approach 1

[0019] Specific embodiment one: the establishment method of the HPLC fingerprint of a kind of Tsinglongyi antioxidant active extract of this embodiment, is to carry out according to the following steps:

[0020] One, the preparation of test solution: a, get Qinglongyi medicinal material, add 10 times of mass percentage and be 95% ethanol, soak for 72h, filter, collect solid phase thing A and liquid phase thing A respectively, solid phase Add 10 times the mass of substance A to 95% ethanol and soak for 48 hours, filter, collect solid phase substance B and liquid phase substance B respectively, and add solid phase substance B to 10 times the mass percent content to be 95% Soak in ethanol for 24 hours, filter, collect the liquid phase C, and then mix the liquid phases A, B and C to obtain the extract;

[0021] b. Concentrate the extract under reduced pressure to a relative density of 1.10 to 1.14 to obtain a concentrated solution, then mix the concentrated solution and water at a...

specific Embodiment approach 2

[0029] Embodiment 2: This embodiment differs from Embodiment 1 in that the gradient elution sequence is: 0min→10min→30min→36min, methanol 35%→45%→60%→60%. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0030] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the microporous membrane filtration in step 1 is performed with a 0.45 μm microporous membrane. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for establishing a high performance liquid chromatography fingerprint spectrum of an Exocarpium Juglandis Immaturum antioxidant active extract, its standard fingerprint spectrum and use. The method includes: 1. preparing a test solution; and 2. making the fingerprint spectrum by injecting the test solution into a high performance liquid chromatographic instrument. The fingerprint spectrum of the Exocarpium Juglandis Immaturum antioxidant active extract involved in the invention has 4 common characteristic peaks, which constitute the fingerprint characteristics of the Exocarpium Juglandis Immaturum antioxidant active extract, and can be taken as the standard fingerprint spectrum of the Exocarpium Juglandis Immaturum antioxidant active extract. According to the application of the Exocarpium Juglandis Immaturum antioxidant active extract standard fingerprint spectrum disclosed in the invention, the Exocarpium Juglandis Immaturum antioxidant active extract fingerprint spectrum can be taken as the standard fingerprint spectrum and used in quality control of Exocarpium Juglandis Immaturum, and is applied in the medical field.

Description

technical field [0001] The invention relates to a method for establishing a high-performance liquid chromatography fingerprint of an anti-oxidation active extract of Qinglongyi. Background technique [0002] The human body will produce a large number of oxygen-containing free radicals in the process of oxidative metabolism. Free radicals will destroy the structure of other molecules through the process of cell transportation, resulting in cell damage, causing the decline of human cell function and aging, which can induce cancer, diabetes, Cardiovascular disease, kidney disease, arteriosclerosis, ischemic heart disease, nervous system disease, eye disease and more than 100 kinds of diseases. Studies have shown that supplementing antioxidant substances can maintain the balance of the production and elimination of free radicals in the body, thereby preventing free radicals from damaging cells and tissues. It is one of the effective measures to prevent various diseases. Eating f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 刘丽娟王艳秋
Owner HEILONGJIANG UNIV
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