Construction method of 1H-NMR (1 hydrogen-nuclear magnetic resonance) fingerprint spectrum of hippocampus, and application

Abstract

The invention discloses a construction method of a 1H-NMR (1 hydrogen-nuclear magnetic resonance) fingerprint spectrum of hippocampus. The method mainly comprises the steps of obtaining the 1H-NMR fingerprint spectrum of the animal medicine hippocampus by an NMR technique, identifying and discriminating chemical components, performing comparison by utilizing a comparison product, performing analysis by combining an NMR spectrum peak chemical shift and a coupling constant, determining a characteristic peak of the fingerprint spectrum of the hippocampus, and characterizing the chemical components contained in the fingerprint spectrum. The method can be used for quality evaluation and quality control of a hippocampus medicine. The method has the benefits that the 1H-NMR fingerprint spectrum of the hippocampus constructed by the NMR technique can simultaneously characterize 20 chemical components, including amino acid, organic acid, alkaloid and the like; the chemical components and the quality characteristic of the hippocampus are relatively comprehensively reflected; and the quality of the hippocampus can be accurately and effectively evaluated and controlled by measuring NMR spectra of different samples and performing analysis and comparison in a chemical structure composition aspect.

Description

technical field [0001] The invention relates to a method for constructing fingerprints of traditional Chinese medicines, in particular to a method for constructing 1H-NMR fingerprints of hippocampus and its application in quality control of hippocampus. Background technique [0002] Hippocampus is a kind of small fish in the near land and shallow sea. As a traditional Chinese medicine, it has strong medical and medicinal value. It can warm the kidney and strengthen Yang, dispel stagnation and reduce swelling. It plays a therapeutic role in classic compound prescriptions such as Guilingji and Compound Zaofan Wan. But at present, the seahorse medicinal material market is chaotic, good and bad are mixed, and there is a lack of reliable quality monitoring methods, which is not conducive to the quality control of raw medicinal materials. [0003] The 2015 edition of "Chinese Pharmacopoeia" contains five kinds of medicinal seahorses, including Hippocampus kelloggi Jordan et Snyder...

AI Technical Summary

Problems solved by technology

[0002] Hippocampus is a kind of small fish in the near land and shallow sea. As a traditional Chinese medicine, it has strong medicinal value. It can warm the kidney and strengthen yang, dispel stagnation and reduce swelling. It plays a therapeutic role in classic compound prescriptions such as Guilingji and compound Zaofan pills. But at present, the market of hippocampal medicinal materials is chaotic, good and bad are intermingled, and there is a lack of reliable quality monitoring methods, which is not conducive to the quality control of raw medicinal materials.

[0003] The 2015 edition of "Chinese Pharmacopoeia" contains five kinds of medicinal seahorses, including Hippocampus histrix Kaup, Hippocampus kuda Bleeker, Hippocampus trimaculatus Leach) and small hippocampus (sea maggot, Hippocampus japonicus Kaup), and the identification method is limited to traits, microscopic identification, lack of specific component research and specific characterization method analysis, there is a certain difficulty in evaluating the quality of medicinal materials

Images