Method for preparing porphyromonas gingivalis culture medium for plaque specimens

A technology for medium preparation and porphyria, which is applied in the field of Porphyromonas gingivalis medium preparation, can solve the problems of low culture separation rate, inability to grow Porphyromonas gingivalis and the like, and achieves the effects of high separation rate and reliable detection.

Inactive Publication Date: 2014-08-27
胡希勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are more than 500 kinds of bacteria living in the human oral cavity, among which more than 300 kinds are colonized in the subgingiva. It is precisely because of this complex sample environment that the culture and isolation rate of P. Streptococcus, Staphylococcus epidermidis, Neisseria, Lactobacillus, Spirochetes, Candida grow faster than P. gingivalis in the current medium and quickly cover the medium, P. gingivalis cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1, the formula for preparing 1000ml of medium contains: 15g of malt extract powder; 150ml of egg white liquid; 2.5ml of hemin solution with a concentration of 5 mg / ml; 0.5ml of vitamin K1 ethanol solution with a concentration of 10mg / ml Magnesium citrate 0.1g; Agar 15g; Coscidin 15mg; Telithromycin 8mg; Linezolid 10mg; Asparagine 3.6g; Preparation method: extract malt powder; egg protein solution; hemin solution; vitamin K1 ethanol solution; magnesium citrate; agar; cocidin; telithromycin; linezolid; asparagine; citric acid tetrahydrate Calcium was dissolved in distilled water, mixed evenly, the distilled water was adjusted to 1000ml, sterilized twice at 85°C for 45 minutes each time, and packed after sterilization.

Embodiment 2

[0013] Example 2, the formula for preparing 1000ml of medium contains: 30g of malt extract powder; 200ml of egg white liquid; 2.5ml of hemin solution with a concentration of 5 mg / ml; 0.5ml of vitamin K1 ethanol solution with a concentration of 10mg / ml Magnesium citrate 0.4g; Agar 20g; Coscidin 20mg; Telithromycin 12mg; Linezolid 5mg; Asparagine 3.6g; Preparation method: extract malt powder; egg protein solution; hemin solution; vitamin K1 ethanol solution; magnesium citrate; agar; cocidin; telithromycin; linezolid; asparagine; citric acid tetrahydrate Calcium was dissolved in distilled water, mixed evenly, the distilled water was adjusted to 1000ml, sterilized twice at 85°C for 45 minutes each time, and packed after sterilization.

Embodiment 3

[0014] Example 3, the formula for preparing 1000ml of medium contains: 15g of malt extract powder; 150ml of egg white liquid; 2.5ml of hemin solution with a concentration of 5 mg / ml; 0.5ml of vitamin K1 ethanol solution with a concentration of 10mg / ml Magnesium citrate 0.1g; Agar 15g; Coscidin 15mg; Telithromycin 8mg; Linezolid 10mg; Asparagine 3.6 g; Amino acid 0.1g; add distilled water to 1000ml. Preparation method: take peeled fresh garlic and wash it, press the juice with a pulverizer and filter to obtain garlic juice; take malt extract powder; egg protein solution; hemin solution; vitamin K1 ethanol solution; magnesium citrate; agar ;Cocidin; Telithromycin; Linezolid; Asparagine; Calcium citrate tetrahydrate; L-cysteine; Garlic squeezed juice was dissolved in distilled water, mixed, distilled water to 1000ml, 85 ℃ intermittent Sterilize twice, 45 min each time, and pack after sterilization.

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Abstract

The invention discloses a method for preparing a porphyromonas gingivalis culture medium for plaque specimens. The method is characterized in that the formula for preparing 1000ml of culture medium contains malt extract, egg protein fluid, heme chloride solution, vitamin K1 ethanol solution, magnesium citrate, agar, synercid, telithromycin, linezolid and asparagines, and distilled water added to 1000ml. The preparation method of the optimized formula comprises the steps of dissolving the malt extract, the egg protein fluid, chlorhematin, agar, magnesium citrate, calcium citrate tetrahydrate, synercid, garlic extract juice and L-cysteine into rhizoma polygonati boiling liquid; evenly mixing, fixing the volume and charging after sterilizing. Compared with the prior art, the porphyromonas gingivalis culture medium has the characteristics of detection reliability and high porphyromonas gingivalis separation rate.

Description

technical field [0001] The present invention is a divisional application, and the parent application is the invention "A Culture Medium for Porphyrobacter gingivalis for Tartar Specimens" filed on July 4, 2012, and the application number is 2012102277749. In particular, it relates to a method for preparing a Porphyrobacter gingivalis culture medium for tartar specimens. Background technique [0002] Periodontitis is a common and frequently-occurring disease in the oral cavity, which belongs to bacterial infectious diseases. Epidemiological studies have shown that Porphyromonas gingivalis (P.gingivalis) is significantly related to the occurrence and development of periodontitis, and is the dominant pathogen of periodontitis. The gingival recession and alveolar bone loss caused by periodontitis in the late stage are generally difficult to regenerate. Therefore, early diagnosis of periodontitis and early detection of P.gingivalis are very important. The current P.gingivalis d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 胡希勇徐蕾刘晓青秦娜
Owner 胡希勇
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