Production technology for extracting oxytetracycline dihyclorate by utilizing hydrochloric acid and oxalic acid
A technology of oxytetracycline base and production process, which is applied in the field of production process of extracting oxytetracycline base, can solve the problems of rising production cost of oxytetracycline base, rising price of oxalic acid, lower competitiveness, etc., and achieve reduction of raw material procurement expenses , Improving market competitiveness and reducing production costs
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Embodiment 1
[0021] Embodiment 1: a kind of production technology that hydrochloric acid and oxalic acid are used to extract oxytetracycline alkali, it comprises the following steps: (1) fermentation; (2) acidification filtration; (3) decolorization crystallization; (4) drying, mixing powder packaging Prepare the finished product of oxytetracycline base;
[0022] Wherein, step (1) fermentation comprises:
[0023] a. Preparation of slant spores: conditions for culturing spores: culture slant spores at 36°C, humidity 55%, culture time 96h;
[0024] b. Primary seed tank cultivation: the cultivation temperature is 30.5°C, the equipment is sealed, and the air is stirred. After about 26 hours of fermentation, it is transferred into the secondary seed tank;
[0025] c. Secondary seed tank cultivation: the cultivation temperature is 30.5°C, mechanical stirring, sterile air, fermentation for 20 hours, and transfer to the third-stage fermenter;
[0026] d. Three-stage fermenter fermentation: the i...
Embodiment 2
[0035] Embodiment 2: a kind of production technology that hydrochloric acid and oxalic acid are used to extract oxytetracycline alkali, it comprises the following steps: (1) fermentation; (2) acidification filtration; (3) decolorization crystallization; (4) drying, mixing powder packaging Prepare the finished product of oxytetracycline base;
[0036] Wherein step (1) fermentation comprises:
[0037] a. Preparation of slant spores: conditions for culturing spores: culture slant spores at 37°C, humidity 60%, culture time 120h;
[0038] b. Primary seed tank cultivation: the cultivation temperature is 31.5°C, the equipment is sealed, and the air is stirred. After about 28 hours of fermentation, it is transferred to the secondary seed tank;
[0039] c. Secondary seed tank cultivation: the cultivation temperature is 31.5°C, mechanical stirring, sterile air, fermentation for 28 hours, and transfer to the third-stage fermenter;
[0040] d. Fermentation in a three-stage fermenter: th...
Embodiment 3
[0049] Embodiment 3: a kind of production technology that hydrochloric acid and oxalic acid are used to extract oxytetracycline alkali, it comprises the following steps: (1) fermentation; (2) acidification filtration; (3) decolorization crystallization; (4) drying, mixing powder packaging Prepare the finished product of oxytetracycline base;
[0050] Wherein, step (1) fermentation comprises:
[0051] a. Preparation of slant spores: culture conditions for spores: culture slant spores at 36.5°C, humidity 58%, culture time 110h;
[0052]b. Primary seed tank cultivation: the cultivation temperature is 31°C, the equipment is sealed, and the air is stirred. After about 27 hours of fermentation, it is transferred to the secondary seed tank;
[0053] c. Secondary seed tank cultivation: the cultivation temperature is 31°C, mechanical stirring, sterile air, fermentation for 24 hours, and transfer to the third-stage fermenter;
[0054] d. Three-stage fermenter fermentation: the inocula...
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