Mangosteen extract and application thereof
A kind of bamboo extract, extract technology, be applied in mangosteen extract and its application field
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Embodiment 1
[0035] Embodiment 1: the concentration of extraction solvent (ethanol) is selected
[0036] Take 20g of dried and crushed mangosteen fruit powder, cold-soak with 200ml of pure water at room temperature, and ultrasonically extract twice, each time for 30min (500W), take the filtrate and combine, recover the solvent under reduced pressure until dry, take an appropriate amount of extract, and prepare 0.05 The mg / ml solution is ready for use.
[0037] Take 20 g of dried and pulverized mangosteen fruit, cold-soak in 200 ml of ethanol with a volume concentration of 30% at room temperature, and ultrasonically extract twice, each time for 30 min (500 W), combine the filtrates, recover the solvent under reduced pressure to dryness, and take an appropriate amount of extract , prepared into a 0.05mg / ml solution for later use.
[0038] Take 20 g of dried and pulverized mangosteen fruit, cold-soak in 200 ml of 70% ethanol at room temperature, and ultrasonically extract twice, each time fo...
Embodiment 2
[0052] Embodiment 2: the selection of solid-liquid ratio:
[0053] Take 20 g of the dried and crushed powder of mangosteen fruit, cold-soak in 160 ml of 70% ethanol at room temperature, and ultrasonically extract twice, each time for 30 min (500 W), take the filtrate and combine, recover the solvent under reduced pressure to dryness, and take an appropriate amount of extract , prepared into a 0.05mg / ml solution for later use.
[0054] Take 20 g of dried and pulverized mangosteen fruit, cold-soak in 200 ml of 70% ethanol at room temperature, and ultrasonically extract twice, each time for 30 min (500 W), combine the filtrates, recover the solvent under reduced pressure to dryness, and take an appropriate amount of extract , prepared into a 0.05mg / ml solution for later use.
[0055] Take 20 g of dried and pulverized mangosteen fruit, cold-soak in 240 ml of ethanol with a volume concentration of 70% at room temperature, and ultrasonically extract twice, each time for 30 min (500...
Embodiment 3
[0061] Embodiment 3: the preparation of mangosteen extract
[0062] Preparation of the crude extract of mangosteen: get 20 g of the dried and pulverized powder of the mangosteen fruit, cold-soak in 200 ml of 70% ethanol at room temperature, and extract by ultrasonic for 2 times, each time for 30 min (500 W), take the filtrates and combine them, and recover the solvent under reduced pressure To dry, the crude extract of mangosteen is obtained, which is used for efficacy research.
[0063] The preparation of mangosteen active enrichment extract: get the powder 100g after the mangosteen fruit is dried and pulverized, cold soak with 70% ethanol 1000ml at room temperature, ultrasonic extraction 2 times, each time 30min (500W), filtrate merges (about 1800ml), decompression Recover the solvent until there is no alcohol smell, pass through AB-8 resin, elute with 30% ethanol, 50% ethanol and 70% ethanol successively, collect each fraction (about 400ml each), evaporate to dryness, and o...
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