Method for identifying resistance of cytosporacarphosperma

A technology of resistance identification and rot disease, applied in application, cultivation, agriculture, etc., can solve the problem that it is difficult to reflect the actual level of resistance of specific materials, achieve rapid and accurate identification and screening, and ensure the chance and reliability of infection with germs Improved effect

Inactive Publication Date: 2014-01-15
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

The detection of pear tree rot disease resistance currently adopts the method of natural investigation in China. Natural investigation will be affected by the test area, test orchard,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 (identification and screening of pear tree rot disease resistance)

[0051] Embodiment 1 carries out as follows:

[0052] 1 Test method

[0053] (1) Cultivation and preparation of pear seedlings: The selected pear seedlings are 4,000 seedlings provided by the Pear Resources Research Group of Zhengzhou Pomology Research Institute, Chinese Academy of Agricultural Sciences. The pear seedlings are cultivated and managed according to conventional soil, fertilizer, water management and pest control measures.

[0054] (2) Strain cultivation: Inoculate pear rot fungus strains stored at low temperature indoors on PSA medium, place them in an incubator at 20°C for 7 days, and then use a 5mm diameter puncher to make fungus cakes for later use; Among them, the formula of PSA medium is: 200g of potatoes, 20g of sucrose, 15-20g of agar, and 1000ml of distilled water.

[0055](3) Pear seedling inoculation: A total of 4,000 seedlings were inoculated from September to Oct...

Embodiment 2

[0071] Embodiment 2 (identification of resistance to rot disease of multiple pear tree varieties)

[0072] 1 Test method

[0073] (1) Cultivation and preparation of pear trees: The selected pear seedlings are 147 pear tree varieties provided by the Pear Resources Research Group of the Zhengzhou Institute of Fruit Trees, Chinese Academy of Agricultural Sciences. Table 1 shows the 147 pear tree varieties. Control measures Cultivation and management of pear seedlings.

[0074] (2) Strain cultivation: Inoculate pear rot pathogen strains stored at low temperature indoors on PSA medium, place them in an incubator at 20°C for 7 days, and then use a 5mm diameter punch to make fungus cakes for later use; Among them, the formula of PSA medium is: 200g of potatoes, 20g of sucrose, 15-20g of agar, and 1000ml of distilled water.

[0075] (3) Pear tree inoculation: A total of 147 varieties were inoculated from September to October of that year. Each variety was connected with 1 tree, each...

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Abstract

The invention relates to a method for identifying the resistance of the cytosporacarphosperma. The method evaluates pear resources and the resistance of a variety through artificial inoculation, solves the problems that high-resistance resource materials in pear disease-resistance breeding are hard to find in the early stage, and rapid evaluation is hard to achieve, and lays the foundation of breeding of the cytosporacarphosperma. The method comprises the following steps: (1) cultivating and preparing pear seedlings, (2) cultivating strains, (3) inoculating the pear seedlings, and (4) inquiring and evaluating the illness state. According to the method, the artificial inoculation technology is adopted, the good epidemic condition is provided for pathogenic bacteria, and the probability that test materials are infected by the pathogenic bacteria is ensured. By the usage of the method, identification and screening of the resistance of the cytosporacarphosperma can be rapidly and accurately carried out, and compared with the result of natural pathogenesis inquiry and identification, the result of the method is improved in accuracy and reliability.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, and in particular relates to a method for identification of pear tree rot disease resistance. Background technique [0002] Pear tree is one of the main fruit tree species in my country. It can be planted in both the north and the south of my country. Its current development area and output rank first in the world. According to statistics, my country's pear tree area is 1 million hectares, with an output of 13 million tons. [0003] During the cultivation of pear trees, there are many kinds of diseases and insect pests, which are seriously harmful. Among them, pear tree rot is the most devastating disease in pear production. This disease is distributed in major pear producing areas in my country, especially in areas or years prone to freezing damage, causing dead trees and dead branches of pear trees to be common and serious. The rate of diseased plants in orchard...

Claims

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Application Information

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IPC IPC(8): A01G17/00
Inventor 周增强侯珲王丽李秀根杨健
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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