Compound tobacco additive as well as preparation method and application
A technology of tobacco additives and compound enzymes, which is applied in the fields of application, tobacco, and tobacco treatment, etc., to achieve the effect of comfortable taste, good market prospect and application value, and transparent and quick aroma
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Embodiment 1
[0014] Take 20 grams of Radix Asparagus, 10 grams of Polygonatum Polygonatum, 10 grams of Tangerine, 40 grams in total, mix well, crush through a 200-mesh sieve, add 400 grams of distilled water, and adjust the pH value to 4.5 with citric acid-sodium citrate buffer solution to obtain Compound Chinese herbal medicine solution. Get 0.08 gram of compound enzyme of cellulase and neutral protease (0.06 gram of cellulase, 0.02 gram of neutral protease), dissolve in 5 ml of distilled water to obtain compound enzyme liquid. Put the compound Chinese herbal medicine solution in a water bath and preheat it to 40°C, add the compound enzyme solution into the compound Chinese herbal medicine solution, carry out enzymatic hydrolysis at a constant temperature for 1 hour, raise the temperature to 80°C, inactivate the enzyme and extract for 2 hours, then filter, and put the filtrate in a water bath at 40°C Concentrate under reduced pressure to obtain 17 grams of fluid paste, which is a composit...
Embodiment 2
[0017] Take 60 grams of Radix Asparagus, 20 grams of Polygonatum Polygonatum, and 20 grams of Tangerine Red, 100 grams in total. After mixing evenly, pulverize and pass through a 200-mesh sieve, add 1500 grams of distilled water, and adjust the pH value to 5.0 with citric acid-sodium citrate buffer solution to obtain Compound Chinese herbal medicine solution. Get 0.4 gram of compound enzyme of cellulase and neutral protease (0.3 gram of cellulase, 0.1 gram of neutral protease), dissolve in 5 ml of distilled water to obtain compound enzyme liquid. Put the compound Chinese herbal medicine solution in a water bath to preheat to 45°C, add the compound enzyme solution into the compound Chinese herbal medicine solution, carry out constant temperature enzymolysis for 1.5 hours, raise the temperature to 80°C, inactivate the enzyme and extract for 2 hours, filter, and put the filtrate in a water bath at 45°C Concentrate under reduced pressure to obtain 42 grams of fluid paste, which is...
Embodiment 3
[0020] Take 80 grams of Radix Asparagus, 40 grams of Polygonatum Polygonatum, 20 grams of Tangerine Red, 140 grams in total, after mixing evenly, pulverize through a 200-mesh sieve, add 2520 grams of distilled water, and adjust the pH value to 5.5 with citric acid-sodium citrate buffer solution to obtain Compound Chinese herbal medicine solution. Get 0.7 gram of compound enzyme of cellulase and neutral protease (0.525 gram of cellulase, 0.175 gram of neutral protease), dissolve in 5 ml of distilled water to obtain compound enzyme liquid. Put the compound Chinese herbal medicine solution in a water bath and preheat it to 50°C, add the compound enzyme solution into the compound Chinese herbal medicine solution, carry out constant temperature enzymolysis for 1.5 hours, raise the temperature to 80°C, inactivate the enzyme and extract for 2 hours, filter, and put the filtrate in a water bath at 50°C Concentrate under reduced pressure to obtain 54 grams of fluid paste, which is a co...
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