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Application of Oleaceran in liver fibroblast proliferation inhibiting drugs

A technology for fibroblasts and drugs, which can be used in drug combinations, pharmaceutical formulations, organic active ingredients, etc., and can solve problems such as deficiency and apoptosis of hepatic stellate cells.

Inactive Publication Date: 2014-03-19
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ideal clinical treatment methods are still lacking. At present, the key to preventing and treating liver fibrosis is to target the links related to the activation of hepatic stellate cells, mainly: reducing liver damage; inhibiting the activation of stellate cells and reducing the production of extracellular matrix; regulating cytokine disorders , promote apoptosis of activated hepatic stellate cells

Method used

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  • Application of Oleaceran in liver fibroblast proliferation inhibiting drugs
  • Application of Oleaceran in liver fibroblast proliferation inhibiting drugs
  • Application of Oleaceran in liver fibroblast proliferation inhibiting drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: the preparation of compound Oleaceran tablet involved in the present invention:

[0011] Take 5 grams of the compound Oleaceran, add 195 grams of dextrin, mix well, and make 1000 tablets by conventional compression.

Embodiment 2

[0012] Embodiment 2: the preparation of compound Oleaceran capsules involved in the present invention:

[0013] Get 5 grams of the compound Oleaceran, add 195 grams of starch, mix well, and make 1000 capsules.

[0014] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example 1

[0015] Experimental Example 1: Using the MTT method to evaluate the inhibitory effect of the compound Oleaceran on liver fibrosis

[0016] 1. Method: NIH / 3T3 cell line was purchased from the cell bank of Shanghai Institute of Cellular Sciences, Chinese Academy of Sciences, cited from ATCC (American Type Culture Collection). Subconfluent NIH / 3T3 cells in the logarithmic growth phase were digested with 0.25% trypsin, washed, centrifuged, and made 1×10 with DMEM medium (containing 10% FCS). 4 cell / ml cell suspension, the survival rate identified by trypan blue staining is greater than 95%, add 100ul per well to a 96-well plate, and store at 37°C, 5% CO 2 After 24 hours of culture and synchronous treatment, the supernatant was discarded, and 200ul of DMEM culture solution (containing 10% FCS) containing different dilutions of drugs was added, cultured for 48 hours, and MTT solution was added to each well for incubation for 4 hours. Discard the culture medium, add 150ulDMSO, shake...

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Abstract

The invention discloses an application of Oleaceran in preparation of liver fibrosis treatment drugs. Pharmacological experiment proves that the Oleaceran can obviously inhibit the occurrence and development of liver fibrosis and can be used for preparing drugs resisting liver fibrosis and preventing liver cirrhosis; the Oleaceran belongs to a brand new skeleton type, has strong inhibitory activity on the liver fibroblast proliferation and has outstanding substantive features; meanwhile, a remarkable progress in obviously realized by applying the Oleaceran to resisting liver fibroblast proliferation.

Description

technical field [0001] The present invention relates to a new application of the compound Oleaceran, in particular to the application of Oleaceran in the preparation of drugs for inhibiting liver fibroblast proliferation. Background technique [0002] Liver fibrosis is a dynamic process from chronic liver injury to liver cirrhosis. In the past 20 years, the research on liver fibrosis has made great progress, and it has been confirmed that liver fibrosis and liver cirrhosis are reversible to a certain extent. However, ideal clinical treatment methods are still lacking. At present, the key to preventing and treating liver fibrosis is to target the links related to the activation of hepatic stellate cells, mainly: reducing liver damage; inhibiting the activation of stellate cells and reducing the production of extracellular matrix; regulating cytokine disorders , promote the apoptosis of activated hepatic stellate cells. [0003] The compound Oleaceran related to the present i...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61P1/16
Inventor 陈军
Owner BINZHOU MEDICAL COLLEGE
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