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Application of Penicillactones C in preparation of drug for inhibiting liver fibroblast proliferation

A fibroblast, liver technology, applied in drug combination, digestive system, pill delivery, etc., can solve problems such as lack of clinical treatment methods

Inactive Publication Date: 2016-04-06
ZIBO DINGLI PATENT INFORMATION CONSULTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some anti-hepatic fibrosis treatments have appeared successively, including chemical drugs, biological agents, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking. The main links are: reduce liver damage; inhibit the activation of stellate cells, reduce the production of extracellular matrix; regulate the disorder of cytokines, promote the apoptosis of activated hepatic stellate cells

Method used

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  • Application of Penicillactones C in preparation of drug for inhibiting liver fibroblast proliferation
  • Application of Penicillactones C in preparation of drug for inhibiting liver fibroblast proliferation
  • Application of Penicillactones C in preparation of drug for inhibiting liver fibroblast proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Preparation of PenicillactonesC tablets of the compound involved in the present invention:

[0014] Take 5 grams of compound PenicillactonesC, add 195 grams of dextrin, mix well, and routinely press into 1000 tablets.

Embodiment 2

[0015] Example 2: Preparation of the compound PenicillactonesC capsules involved in the present invention:

[0016] Take 5 grams of compound PenicillactonesC, add 195 grams of starch, mix well, and pack into capsules to make 1000 capsules.

[0017] The pharmacodynamics experiment will further illustrate its drug activity below.

experiment example 1

[0018] Experimental example 1: MTT method was used to evaluate the inhibitory effect of the compound PenicillactonesC on liver fibrosis

[0019] 1. Methods: The NIH / 3T3 cell line was purchased from the cell bank of the Shanghai Institute of Cell Research, Chinese Academy of Sciences, which was quoted from ATCC (American Type Culture Collection). Subconfluent NIH / 3T3 cells in logarithmic growth phase were digested with 0.25% trypsin, washed, centrifuged, and made into 1×10 cells in DMEM medium (containing 10% FCS). 4 cell / ml cell suspension, trypan blue staining to identify the viability of more than 95%, add 100ul per well to a 96-well plate, at 37°C, 5% CO 2 After 24 hours of synchronous treatment, the supernatant was discarded, 200 ul of DMEM culture medium (containing 10% FCS) containing drugs at different dilution concentrations was added, and 48 hours of culture were added, and MTT solution was added to each well to incubate for 4 hours. Discard the culture medium, add 1...

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PUM

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Abstract

The invention discloses application of Penicillactones C in preparation of a drug for treating liver fibrosis. A pharmacological test proves that the Penicillactones C can obviously inhibit the occurrence and development of liver fibrosis, and can be used for preparing the drug for resisting liver fibrosis and preventing and treating liver cirrhosis; the Penicillactones C belongs to a brand new skeleton type, has strong inhibitory activity to liver fibroblast proliferation, and has outstanding substantive distinguishing features, and a remarkable improvement is obviously achieved when the Penicillactones C is used for resisting liver fibroblast proliferation.

Description

technical field [0001] The invention relates to a new use of the compound PenicillactonesC, in particular to the application of PenicillactonesC in the preparation of a drug for inhibiting the proliferation of liver fibroblasts. Background technique [0002] Liver fibrosis is a dynamic process from chronic liver injury to liver cirrhosis. In the past 20 years, the research on liver fibrosis has made great progress, and it has been confirmed that liver fibrosis and a certain degree of liver cirrhosis are reversible. In recent years, some anti-hepatic fibrosis treatment methods have emerged one after another, including chemical drugs, biological preparations, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking. The main steps are: alleviating liver injury; inhibiting stellate cell activation, reducing extracellular matrix production; regulating cytokine disorder and promoting apoptosis of activated hepatic stellate ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P1/16
CPCA61K31/365A61K9/2059A61K9/4866
Inventor 田丽华
Owner ZIBO DINGLI PATENT INFORMATION CONSULTING CO LTD
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