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Method for detecting cadmium of enzyme preparation

A detection method and enzyme preparation technology, which are used in the detection field of cadmium in enzyme preparations

Inactive Publication Date: 2014-04-02
WUHAN SUNHY BIOLOGICAL
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Problems solved by technology

In addition to the above mentioned standards involving the determination of cadmium in enzyme preparations, there are no relevant detection standards. In most cases, similar standards can only be used for determination. Therefore, in order to better promote the development of enzyme preparations, it is urgent to establish various A detection method of hygienic quality standard

Method used

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  • Method for detecting cadmium of enzyme preparation
  • Method for detecting cadmium of enzyme preparation
  • Method for detecting cadmium of enzyme preparation

Examples

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Embodiment 1

[0042] Example 1 Determination of cadmium in enzyme preparations by graphite furnace atomic absorption spectrometry

[0043] The cadmium in the sample turns into cadmium ions under the action of acid, precipitates and filters to remove the precipitate, dilutes to a constant volume, injects it into the graphite furnace of the atomic absorption spectrophotometer, absorbs the 228.8nm resonance line after electrothermal atomization, and in a certain concentration range, Its absorption value is directly proportional to the cadmium content, and it is quantitative compared with the standard series.

[0044] (1) Reagents and solutions

[0045] Nitric acid, superior grade. Perchloric acid, superior grade. Mixed acid: Mix 4 volumes of nitric acid with 1 volume of perchloric acid. Cadmium standard stock solution: GSB04-1721-2004, 1000μg / ml, national standard sample, purchased from National Nonferrous Metals and Electronic Materials Analysis and Testing Center. Cadmium standard interm...

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Abstract

The invention relates to the technical field of detection of biological preparations, in particular to a method for detecting cadmium of an enzyme preparation. The method comprises the following steps: (1) pretreating a sample and performing wet digestion on the sample; (2) preparing a standard curve; (3) measuring; (4) calculating the result. The method is verified through accuracy degree and precision degree (repeatability and reproducibility), the applicability of the enzyme preparations in different industries and the true value regression test of standard rice substances, and the method is a simple, convenient, reliable and accurate method.

Description

technical field [0001] The invention relates to the technical field of biological agent detection, in particular to a method for detecting cadmium in enzyme preparations. Background technique [0002] Enzyme preparation is a highly specific biocatalyst, which is widely used in food, textile, feed, medicine, papermaking and other industries. In recent years, the enzyme preparation industry has developed rapidly, and the variety of enzyme preparation products has continued to increase. It is not yet perfect, and the detection methods of various hygiene and quality indicators are relatively backward. [0003] For example, the detection of cadmium in feed enzyme preparations is specified in NY / T722-2003 "General Rules for Feed Enzyme Preparations" to use GB / T13082-1991 "Determination of Cadmium in Feed", but this standard is applicable to the determination of cadmium in feed, The cadmium content in the enzyme preparation is low, and the results obtained by using this method are...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28
Inventor 詹志春徐丽王冠
Owner WUHAN SUNHY BIOLOGICAL
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