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A method of improving soil by using phosphate solubilizing bacteria

A technology of soil improvement and phosphorus solubilizing bacteria, applied in the restoration of polluted soil, botanical equipment and methods, and applications, can solve the problems of restricting the growth of surface vegetation and ecological reconstruction, the reduction of biological communities, and soil desertification

Active Publication Date: 2016-06-15
CHINA UNIV OF MINING & TECH (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The disturbance of coal mining causes the fertility reduction of the surface soil, the destruction of the soil structure, and the sharp reduction of the biological community. In addition, China's coal is mainly distributed in the arid and semi-arid regions of the west. The region is arid and water-deficient, the soil desertification is serious, and the background value of available nutrients in the soil is low. , the phenomenon of phosphorus deficiency is serious, which seriously restricts the growth of surface vegetation and ecological reconstruction in this area.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the impact analysis of the phosphate-solubilizing bacteria of different usage amounts on the germination rate of plant seeds

[0044] The phosphate-solubilizing bacterium used in this example is Pantoea stevii CA, and the seeds of the tested plants are the seeds of the corn variety Zhongnuo No. 1 (certification number: 0103006-2000, Beijing Zhongnongzuo Technology Development Co., Ltd.).

[0045] 1. Cultivation of Pantoea stevii CA

[0046] Montkina Medium: (NH 3 ) 2 SO 4 0.5g, NaCl0.3g, KCl0.3g, MgSO 4 0.3g, MnSO 4 0.03g, FeSO 4 0.03g, Ca(PO 4 ) 2 5g, glucose 10g, distilled water 1000ml, pH7.0-7.5.

[0047] Inoculate Pantoea stewartii CA into Montina medium, shake and culture at 30°C with a shaker rotation speed of 160r / min, after 7 days of cultivation, make Pantoea steviae CA in logarithmic growth phase, and set aside. It was determined that the OD440 of Pantoea stewari CA in the culture medium in the logarithmic growth phase was 0.84.

[0048]...

Embodiment 2

[0056] Embodiment 2, the effect analysis of the phosphorus-solubilizing bacteria of different usage amounts on soil improvement

[0057] Soil for test: collected from the subsidence of Gaojiapan Huojitu Mining Area, Daliuta Town, Shenmu County, Shaanxi Province. Soil samples were sterilized by high-temperature and high-pressure steam (121°C, 1h), air-dried, and passed through a 1mm sieve for later use. It has been determined that the content of available phosphorus (phosphorus that can be directly absorbed by plants) in the soil in the subsidence area is 7.2mg / kg soil, and available potassium (potassium in the soil that can be absorbed by crops in the current season, including water-soluble potassium and exchangeable potassium) The content is 50mg / kg soil (note: it refers to the available potassium per kg dry weight soil, and the expression of available phosphorus is the same), the pH value is 7.60, the conductivity is 60μs / cm, and the maximum water holding capacity is 25%. W...

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Abstract

The invention discloses a method for improving soil by virtue of phosphate solubilizing bacteria. The method comprises the step of adding the phosphate solubilizing bacteria into to-be-improved soil, wherein the adding amount of the phosphate solubilizing bacteria in each kilometer of lean soil by dry weight according to that the CFU (colony-forming unit) number of the added phosphate solubilizing bacteria is equal to that of 100ml of 10% phosphate solubilizing bacteria, and the 10% phosphate solubilizing bacteria is obtained by diluting a culture solution of the phosphate solubilizing bacteria in a logarithmic phase with distilled water by 10 time. Experiments prove that by improving the lean soil by utilizing the phosphate solubilizing bacteria by virtue of the method disclosed by the invention, the germination rate, plant height, first-leaf expanded width, first-leaf expanded length, root length, root tip number and the like of corn plants are obviously promoted. The method has a positive ecological significance to the improvement of the land rehabilitation efficiency and provides a microbial technical support to the land rehabilitation and ecological reconstruction of a mine area.

Description

technical field [0001] The invention relates to a method for improving soil by using phosphorus solubilizing bacteria. Background technique [0002] Phosphorus is one of the three essential elements for plant growth and is an important material basis for plant growth. The amount of phosphorus absorbed by plants is significantly positively correlated with its biomass and yield. There is a large amount of phosphorus in the soil, but most of these phosphorus elements are organic and inorganic phosphorus that cannot be directly absorbed by plants. Phosphorus solubilizers are a special functional group of microorganisms that can convert insoluble phosphorus into soluble phosphorus that plants can absorb and utilize in soil. For the calcareous soil in the north, the phosphorus solubilizing effect of phosphate solubilizing bacteria is more obvious. Phospholytic bacteria can secrete a variety of organic acidic substances or accompanied by respiration or assimilation of NH 4 + H ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10A01G1/00A01C1/00
Inventor 毕银丽李少朋全文智高斐
Owner CHINA UNIV OF MINING & TECH (BEIJING)
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