Method for rapidly growing and cultivating arabidopsis thaliana

An Arabidopsis thaliana, rapid technology, applied in the directions of botanical equipment and methods, cultivation, culture medium, etc., can solve the problems of difficulty in maintaining soil moisture and soil temperature, low survival rate of Arabidopsis thaliana, affecting flowering and fruiting, etc. The number of pods is large, the value of wide application and promotion, and the effect of neat leaves

CN109496704AActive Publication Date: 2019-03-22XINJIANG UNIVERSITY +1
7 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-03-22

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

Aiming at the problems that the survival rate of arabidopsis thaliana is not high, plants are weak and small, and later blossoming and fruiting are influenced caused for the reason that in the later period of existing arabidopsis thaliana soil culture methods or transplanting methods, the soil humidity and the soil pH are difficult to keep, the phenomenon that the plants are withered due to the fact that the water loss is too fast occurs frequently, or vermiculite becomes sticky after too much water is watered, the permeability is reduced roots are rotten and root growth vigor is reduced due to a large amount of propagation of fungi and algae the invention provides a technical method for rapidly growing and cultivating arabidopsis thaliana, a culture device is transformed on the basis thatthe arabidopsis thaliana is cultured by mixed soil, and a technical method capable of keeping certain temperature and humidity of the soil is established, so that the growth state of the cultured arabidopsis thaliana is good, and the growth and development speed of the arabidopsis thaliana is remarkably increased, the plants are high, the number of rosette leaves is large, the number of pods is large, and the method has a wide application and popularization value.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention belongs to the field of biotechnology, specifically, the invention belongs to the technical field of rapid growth and cultivation of Arabidopsis thaliana. Background technique:

[0002] Arabidopsis thaliana (Arabidopsis thaliana) is a dicotyledonous model plant belonging to the Brassicaceae Arabidopsis genus. It has the characteristics of self-pollination, relatively distinct morphological characteristics, small individual plants, short growth period, and small genome. It is the first to complete Higher plants whose entire genome sequences have been determined have become widely recognized model plants in various research fields. Generally, Arabidopsis thaliana can complete the entire life cycle in 90-120 days. Arabidopsis seedlings are weak and vulnerable to damage. Nutrients, light, temperature, water and air humidity have a relatively large impact on the growth of Arabidopsis. Since many domestic units lack the conditions for Arabidop...

Examples

Embodiment 1

[0032] Embodiment one: Arabidopsis rapid growth cultivation method

[0033] (1) Seed pretreatment: Arabidopsis thaliana seeds were treated with 10% sodium hypochlorite (900 μL absolute ethanol + 100 μL sodium hypochlorite) for 5 minutes while shaking continuously; after sucking out the sodium hypochlorite, wash 3-5 times with absolute ethanol. After volatilizing the ethanol in the clean seeds in the ultra-clean workbench, it can be used for subsequent experiments;

[0034] (2) Planting on MS solid medium: spot the seeds on MS solid medium without antibiotics, vernalize at 4°C for 2-3 days, light for 6 hours, cultivate in the dark for 1-2 days, place them in light for 14:8 hours, 22 Cultivate in Arabidopsis thaliana culture room at ℃±1℃ for 15 days;

[0035] (3) Transplanting and planting treatment: put the mixed nutrient soil into the seedling pot, spread the tin foil paper on the seedling pot, punch holes, and transplant the Arabidopsis seedlings prepared in step (2) into the ...

Embodiment 2

[0042] Example 2: Effects of different covering materials on the growth of Arabidopsis thaliana

[0043] 1. Method

[0044] (1) Seed treatment Arabidopsis thaliana seeds were treated with 10% sodium hypochlorite (900 μL absolute ethanol + 100 μL sodium hypochlorite) for 5 minutes while shaking continuously; after sucking out the sodium hypochlorite, they were washed 3-5 times with absolute ethanol. After volatilizing the ethanol in the clean seeds in the ultra-clean workbench, it can be used for subsequent experiments

[0045] (2) MS solid medium culture Spot the seeds on MS solid medium without antibiotics, vernalize at 4°C for 2-3d, light for 6h, dark culture for 1-2d, place under light for 14:8h, 22°C The Arabidopsis culture room was cultured at ±1°C for 15 days.

[0046] (3) Transplanting and colonization treatment After Arabidopsis seedlings were cultured in MS solid medium for 15 days, the seedlings were moved to a mixed medium with a volume ratio of perlite: vermiculi...

Embodiment 3

[0065] Example 3: Effects of different culture methods on the growth of Arabidopsis thaliana using tinfoil

[0066] 1. Method

[0067] Set up 4 treatment groups, be respectively conventional soil culture method, the tinfoil paper covering method provided by the application, conventional hydroponics method and tinfoil paper covering hydroponics method.

[0068] (1) Seed treatment

[0069] Treat Arabidopsis thaliana seeds with 10% sodium hypochlorite (900 μL absolute ethanol + 100 μL sodium hypochlorite) for 5 minutes, and shake continuously during the period; after sucking out the sodium hypochlorite, wash with absolute ethanol for 3-5 times, and volatilize the seeds in an ultra-clean workbench The ethanol can be used for subsequent experiments. The seeds are placed in MS solid medium without antibiotics and vernalized at 4°C for 2-3 days.

[0070] (2) planting

[0071] ① Conventional soil cultivation method: mix perlite: vermiculite: flower-soil volume ratio 1:1:3 evenly, s...