Method for rapidly growing and cultivating arabidopsis thaliana
An Arabidopsis thaliana, rapid technology, applied in the directions of botanical equipment and methods, cultivation, culture medium, etc., can solve the problems of difficulty in maintaining soil moisture and soil temperature, low survival rate of Arabidopsis thaliana, affecting flowering and fruiting, etc. The number of pods is large, the value of wide application and promotion, and the effect of neat leaves
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Embodiment 1
[0032] Embodiment one: Arabidopsis rapid growth cultivation method
[0033] (1) Seed pretreatment: Arabidopsis thaliana seeds were treated with 10% sodium hypochlorite (900 μL absolute ethanol + 100 μL sodium hypochlorite) for 5 minutes while shaking continuously; after sucking out the sodium hypochlorite, wash 3-5 times with absolute ethanol. After volatilizing the ethanol in the clean seeds in the ultra-clean workbench, it can be used for subsequent experiments;
[0034] (2) Planting on MS solid medium: spot the seeds on MS solid medium without antibiotics, vernalize at 4°C for 2-3 days, light for 6 hours, cultivate in the dark for 1-2 days, place them in light for 14:8 hours, 22 Cultivate in Arabidopsis thaliana culture room at ℃±1℃ for 15 days;
[0035] (3) Transplanting and planting treatment: put the mixed nutrient soil into the seedling pot, spread the tin foil paper on the seedling pot, punch holes, and transplant the Arabidopsis seedlings prepared in step (2) into the ...
Embodiment 2
[0042] Example 2: Effects of different covering materials on the growth of Arabidopsis thaliana
[0043] 1. Method
[0044] (1) Seed treatment Arabidopsis thaliana seeds were treated with 10% sodium hypochlorite (900 μL absolute ethanol + 100 μL sodium hypochlorite) for 5 minutes while shaking continuously; after sucking out the sodium hypochlorite, they were washed 3-5 times with absolute ethanol. After volatilizing the ethanol in the clean seeds in the ultra-clean workbench, it can be used for subsequent experiments
[0045] (2) MS solid medium culture Spot the seeds on MS solid medium without antibiotics, vernalize at 4°C for 2-3d, light for 6h, dark culture for 1-2d, place under light for 14:8h, 22°C The Arabidopsis culture room was cultured at ±1°C for 15 days.
[0046] (3) Transplanting and colonization treatment After Arabidopsis seedlings were cultured in MS solid medium for 15 days, the seedlings were moved to a mixed medium with a volume ratio of perlite: vermiculi...
Embodiment 3
[0065] Example 3: Effects of different culture methods on the growth of Arabidopsis thaliana using tinfoil
[0066] 1. Method
[0067] Set up 4 treatment groups, be respectively conventional soil culture method, the tinfoil paper covering method provided by the application, conventional hydroponics method and tinfoil paper covering hydroponics method.
[0068] (1) Seed treatment
[0069] Treat Arabidopsis thaliana seeds with 10% sodium hypochlorite (900 μL absolute ethanol + 100 μL sodium hypochlorite) for 5 minutes, and shake continuously during the period; after sucking out the sodium hypochlorite, wash with absolute ethanol for 3-5 times, and volatilize the seeds in an ultra-clean workbench The ethanol can be used for subsequent experiments. The seeds are placed in MS solid medium without antibiotics and vernalized at 4°C for 2-3 days.
[0070] (2) planting
[0071] ① Conventional soil cultivation method: mix perlite: vermiculite: flower-soil volume ratio 1:1:3 evenly, s...
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