Bacillus and application thereof

A technology of bacillus pumilus and bacteria agent, applied in the field of bacillus and its application, can solve the problems of effective nutrients restricting the development of tobacco industry, etc., achieve good application value, high solubility, and promote plant growth

Active Publication Date: 2021-12-17
LIANGSHAN BRANCH OF SICHUAN TOBACCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the content and supply of phosphorus and potassium elements in the soil in the tobacco area have a great impact on the gr

Method used

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  • Bacillus and application thereof
  • Bacillus and application thereof
  • Bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Isolation and Identification of Bacillus pumilus PSB25

[0044] 1.1 Selection of tobacco plants

[0045] The roots of the tobacco in this example were collected from Puji Town, Puge County, Liangshan Prefecture, Sichuan Province. A number of well-growing tobacco leaf plants were randomly collected, put into sterilized sealed plastic bags, labeled, brought back to the laboratory in an ice box in time, and stored in a 4°C refrigerator.

[0046] 1.2 Isolation of Phosphorus-Solubilizing Endophytic Bacteria from Tobacco

[0047] Configure inorganic phosphorus medium and organic phosphorus medium. Sterilize the surface of 5g of tobacco root samples stored in a refrigerator (4°C), transfer them to a sterile mortar and grind them thoroughly, apply the grinding solution on inorganic phosphorus medium and organic phosphorus medium plates, and culture at a constant temperature of 30°C for 4-5 days. day, select the strain with hydrolysis circle ( figure 1 , Table 1) w...

Embodiment 2

[0056] Example 2 Endophytic bacteria produce indole acetic acid (IAA) assay

[0057] The 17 strains isolated above were cultured to the logarithmic phase, and 50 μl of the bacterial solution was placed in beef extract liquid medium (about 3 mL) containing 0.5 g / L tryptophan (referring to each bacterial solution corresponding to one containing 0.5 g / L tryptophan beef extract liquid medium), repeat 3 times, shake culture at 28°C 140r / min for 1.5 days, take 50µl on a clean white porcelain plate, add 100µL color reagent, and keep away from light for 30min at 25°C , if there is pink, it shows that IAA is produced, and the beef extract liquid medium containing 0.5g / L tryptophan without inoculation is used as a control.

[0058] Continue to culture the endophytic bacteria producing indole acetic acid in beef extract liquid medium containing 0.5g / L tryptophan for 24-48h, centrifuge at 8000r / min for 5min, take 2ml of the supernatant and add 4ml of Salkowski chromogenic reagent, After...

Embodiment 3

[0062] Embodiment 3 Potassium solution ability is measured

[0063] Determination of the potassium-dissolving and growth-promoting ability of the phosphorus-dissolving bacterial strains, the results show that the potassium-dissolving effects of the 17 phosphorus-dissolving bacterial strains are all significant (Table 3), and the potassium-dissolving rates are all between 0.20% and 0.29%. Potassium rates are above 0.25%, respectively 0.27% and 0.29%.

[0064] Table 3 Determination of Potassium Solubilizing Ability of Phosphorus Solubilizing Bacteria

[0065]

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Abstract

The invention discloses bacillus and application thereof. The bacillus is bacillus pumilus PSB25, the preservation number is CCTCC (China Center For Type Culture Collection) NO: M2020851, and a 16SrRNA (Ribonucleic Acid) gene sequence is SEQ ID NO: 1. The bacillus pumilus PSB25 can promote the growth of corn plants, increase the root number, the root length, the plant height, the leaf number and the leaf area of corn, inhibit phytopathogen and increase the phosphorus content in the plants, and has a good application prospect in the improvement of the phosphorus content in tobacco leaves and the growth of the plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a bacillus and an application thereof. Background technique [0002] Tobacco is an important economic crop in my country. In addition to being used as a raw material for cigarettes, a large amount of natural chemical substances contained in it, such as nicotine, protein, solanesol, and nicotine, are high-quality chemical and pharmaceutical raw materials. Mining microbial agents suitable for tobacco cultivation can effectively improve the yield and quality of tobacco leaves. Phosphorus is closely related to the photosynthesis of crops. Phosphorus is involved in the process of forming compounds in photosynthesis, and it is also involved in the synthesis of sucrose and starch. Phosphorus is also indispensable for the synthesis of nicotine and oil in tobacco. Therefore, the content and supply of phosphorus and potassium elements in the soil of the smoking area have a great impact on the g...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/60A01N63/22A01P21/00A01P1/00A01P3/00C12R1/07
CPCC12N1/20C05G3/00C05G3/60A01N63/22
Inventor 陈玉蓝向泉桔王勇辜运富罗琳陈强赵珂
Owner LIANGSHAN BRANCH OF SICHUAN TOBACCO
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