Ophiocordyceps sinensis strain with high spore yield

A technology of Cordyceps sinensis fungi and strains, applied in the directions of fungi, methods of using spores, microorganisms, etc.

Inactive Publication Date: 2014-05-14
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The strain has been preserved in the General Microorganism Center of China Committ

Method used

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  • Ophiocordyceps sinensis strain with high spore yield
  • Ophiocordyceps sinensis strain with high spore yield
  • Ophiocordyceps sinensis strain with high spore yield

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1, the separation of Cordyceps sinensis 1621 and its biological characteristics

[0033] 1. Isolation of Cordyceps sinensis 1621

[0034] The inventor of the present application screened the strains from the five provinces and regions of the country's Cordyceps sinensis production area from the identified Cordyceps strain bank in the laboratory, and obtained the dominant bacterial strain 1621 bacteria. After liquid culture, its mycelium dry weight and medium The test performance of the two indicators of utilization rate (residual sugar content) was good; after solid culture, the test performance of 1621 bacteria producing conidia was good. For the test method, see [Yao Yijian, Ren Shuyu. A method for the preparation of conidia cuttings of Cordyceps sinensis and the dynamic counting method for sporulation. Patent application number: 201110055807.1; Yao Yijian, Ren Shuyu, Wang Wenjing. A method for the preparation of conidia coverslips of Cordyceps sinensis a...

Embodiment 2

[0043] The sporulation situation of embodiment 2, Cordyceps sinensis 1621

[0044] The strains in this example: Cordyceps sinensis 1621 and Cordyceps sinensis 762

[0045] 1. Preparation of the seed solution

[0046]Cordyceps sinensis was inoculated on the above solid medium and cultured at 15° C. for 90 days to obtain the Cordyceps sinensis strain. Wash the Cordyceps fungus strain plate with sterile water, and inoculate the washed bacterial suspension in the above-mentioned liquid medium with an inoculation amount of 20% by volume, and the bottling amount is 1 / 5 of the volume of the Erlenmeyer flask, and then at 18°C, The rotating speed is 100rpm, and the shaking culture is carried out for 14 days under 24 hours of dark conditions to obtain the first strain of Cordyceps sinensis; the first strain of Cordyceps sinensis is inoculated in a new liquid medium with an inoculation amount of 10% by volume, and other cultures The conditions are the same, and the second strain liquid...

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Abstract

The invention discloses an Ophiocordyceps sinensis strain with high spore yield. The Ophiocordyceps sinensis strain with high spore yield provided by the invention is Ophiocordyceps sinensis 1621, the registered number of which in the China General Microbiological Culture Collection Center is CGMCC No.6445. The Ophiocordyceps sinensis 1621 has a fast mycelium growth speed, short conidiospore generation time and high spore yield.

Description

technical field [0001] The invention relates to a strain of Cordyceps sinensis with high sporulation production. Background technique [0002] Cordyceps sinensis [Ophiocordyceps sinensis (Berk.) G. H.Sung, J.M.Sung, Hywel-Jones & Spatafora (≡Cordyceps sinensis (Berk.) Sacc.)] is a rare medicinal fungus unique to the Qinghai-Tibet Plateau. Modern research shows that Cordyceps sinensis has a variety of pharmacological effects and has a good application prospect. Although a large amount of research work has been done on Cordyceps sinensis, there are still obvious technical difficulties in artificial cultivation so far. Solving these problems requires a more comprehensive grasp of the biological traits of Cordyceps sinensis. Therefore, the innovation of biological research technology of Cordyceps sinensis has important theoretical and practical significance. [0003] In the research on the cultivation of Cordyceps sinensis, the research on conidia is closely related to the pr...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
Inventor 姚一建任蜀豫王晓亮
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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