A method for high-efficiency and rapid propagation of excellent strains of Saltwort
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It is an excellent technology of Saltwort, applied in the field of high-efficiency and rapid propagation of excellent strains of Saltwort, which can solve the problems of browning, pollution, and high heterozygosity of seeds, and achieve the maintenance of excellent traits, low germination rate, and few experimental materials Effect
What is AI technical title?
AI technical title is built by PatSnap AI team. It summarizes the technical point description of the patent document.
It is an excellent technology of Saltwort, applied in the field of high-efficiency and rapid propagation of excellent strains of Saltwort, which can solve the problems of browning, pollution, and high heterozygosity of seeds, and achieve the maintenance of excellent traits, low germination rate, and few experimental materials Effect
CN104082141BActive 📅 Publication Date: 2016-10-26HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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[0031] The present invention will be further described in detail according to the examples below.
[0032] A kind of method for high-efficiency and rapid propagation of excellent strains of A. chinensis in the field, specifically as follows:
[0033] 1) Get the root of the 2-5cm long to-be-propagated Salmonella plant, and bury it in 2-5cm thick soil to raise seedlings.
[0034] 2) Get the leaves of the non-lignified seedlings obtained through root propagation 25-40d, and carry out surface disinfection ( figure 1 );
[0035] 3) Cut the leaves into 0.5×0.5cm 2 Cut pieces with veins of large and small sizes were inoculated on cluster bud induction medium (MS+0.5~4.0mg / L TDZ+30g / L sucrose+7.0g / L agar), and emerald green adventitious bud clusters were obtained after 3 to 4 weeks; Cluster buds were transferred to cluster bud proliferation and elongation medium (MS+0.2~0.5mg / L TDZ+0.01~0.3mg / L IBA or NAA+30g / L sucrose+7.0g / L agar); 2.0~3.0cm, transferred to the rooting medium 91 / ...
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Abstract
The invention discloses a high-efficiency rapid propagation method of good Rhus chinensis strains. The high-efficiency rapid propagation method comprises the following steps: performing root-pressing propagation on the good field perennial Rhus chinensis earth surface root and culturing for 25-40 days to grow a complete plant; carrying out surface sterilization on leaves of the seedling of the complete plant, taking leaf vein and inoculating on an induction medium, performing illumination culture for 28-35 days to enable the two ends of the leaf vein to grow adventitious buds; inoculating the adventitious buds on a multiple-shoot proliferation and extension medium for bud proliferation and extension, transferring to a rooting medium for rooting after buds grow for 2-3cm, thereby obtaining the strong Rhus chinensis test-tube plantlet. The high-efficiency rapid propagation method has the advantages that root-pressing propagation is adopted to strength the seedling, the problem of pollution and browning severity caused by a situation that field materials are directly drawn as the explants is solved, and the method is convenient for realizing regeneration and obtaining tissue culture seedlings.
Description
technical field [0001] The invention relates to a method for high-efficiency and rapid propagation, especially a method for high-efficiency and rapid propagation of excellent strains of A. chinensis. Background technique [0002] Saltwood is a small deciduous tree belonging to the family Anacardiaceae. It has strong adaptability and wide distribution. Its seeds contain a large amount of unsaturated fatty acids, and its oil has high edible value. It is also a potential tree species for bio-energy raw material oil. Oil, ornamental and raw materials for industrial dyes, etc. Saltwood has a high medicinal value, and gallnuts formed by aphid galls parasitic on its leaves are important traditional Chinese medicinal materials and industrial raw materials, and are important forest special products in my country. With the continuous increase of the demand for the related products of Saltwort, it is of great significance to cultivate high-yield and high-quality Saltwort varieties and...
Claims
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