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Method For Illuminating An Object In Digital Light Microscope, Digital Light Microscope And Bright Field Reflected-light Illumination Device For Digital Light Microscope

A lighting device and digital optics technology, applied in microscopes, optics, optical components, etc., can solve problems such as unsatisfactory lighting efficiency, and achieve cost-effective, space-saving, and fast image acquisition effects

Inactive Publication Date: 2014-10-22
CARL ZEISS MICROSCOPY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Due to the variety of optical components in the illumination beam path of Kohler illumination, the efficiency of illumination (especially in the case of brightfield illumination) is often unsatisfactory despite the use of LEDs

Method used

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  • Method For Illuminating An Object In Digital Light Microscope, Digital Light Microscope And Bright Field Reflected-light Illumination Device For Digital Light Microscope
  • Method For Illuminating An Object In Digital Light Microscope, Digital Light Microscope And Bright Field Reflected-light Illumination Device For Digital Light Microscope

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Embodiment Construction

[0038]figure 1 A first preferred embodiment of a bright field reflected light illumination device according to the invention in a Nelson configuration or so-called "critical" illumination is shown. The device comprises at least one LED 01 as light source, which LED is equipped with a corresponding optical assembly as light collector 02 . The light emitted by the LED 01 is conjugated with respect to the aperture stop 10 in the illumination beam path through the plane 03 via the intermediate optical unit 04 into the homogenizer embodied as a light mixing rod 06 . In the conjugate plane 03, in an alternative embodiment, a variable second aperture stop can be used in order to be able to set the illumination and observation apertures independently of each other. In particular, a contrast enhancement is thus achieved.

[0039] Ideally, an image of the light source (ie, the emitting LED chip) in the illustrated embodiment appears at the entrance of the homogenizer. However, the ima...

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Abstract

The invention relates to a method for illuminating an object in a digital light microscope, to a digital light microscope, and to a bright field reflected-light illumination device for a digital light microscope. According to the invention, the bright field reflected-light illumination and the dark field reflected-light illumination are configured with light-emitting diodes as light sources and are individually or jointly drivable via a control unit. Both the bright field reflected-light illumination and the dark field reflected-light illumination are configured as “critical” illumination, in which the light source is imaged into the object plane.

Description

technical field [0001] The present invention relates to a method for illuminating an object in a digital optical microscope, a digital optical microscope and a coaxial bright field reflected light illumination device for a digital optical microscope. Background technique [0002] Different illumination strategies for optical microscopy are known from the prior art. [0003] First, a distinction is made between transmitted light microscopy and reflected light microscopy. Especially in reflected light microscopy, the sample is illuminated from the direction of the objective. For this purpose, so-called Kohler illumination has been used for a long time, whereby the aperture and the diameter of the illuminated object can be influenced independently of each other. In this case, light from a light source is directed through an illuminated field diaphragm into an area where multiple color and rejection filters can be inserted. The light then passes through the aperture stop and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/06
CPCG02B21/125G02B21/082G02B21/084
Inventor 汉斯·谭德乐约翰内斯·诺布里奇多米尼克·斯特尔亚历山大·伽杜克恩里科·盖勒简·布克海斯特马克思·冯科
Owner CARL ZEISS MICROSCOPY GMBH
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