Method for feeding a large number of laboratory test populations of Opisina arenosella Walkers

Abstract

The invention belongs to the technical field of insect feeding and particularly discloses a method for feeding a large number of laboratory test populations of Opisina arenosella Walkers. The method is an improvement on an existing method for feeding the Opisina arenosella Walkers according to mating and oviposition characteristics of the Opisina arenosella Walkers. Mating space is enlarged, and the number of matched pairs of male and female Opisina arenosella Walkers is increased, so that female Opisina arenosella Walkers are stimulated to oviposit. In addition, the number of places where the female Opisina arenosella Walkers can oviposit is increased, the oviposition area is enlarged, and the survival rate of ova is increased. Tedious procedures of collecting ova through independent test tubes and leaf replacement during larva feeding are simplified, workload is decreased greatly, and the large number of standard test populations of Opisina arenosella Walkers with a high survival rate and simplified procedures can be obtained truly.

Description

technical field [0001] The invention relates to the technical field of insect rearing, more particularly, to a method for raising a large number of indoor experimental populations of the weaver moth. Background technique [0002] Coconut Weaver Moth ( Opisina arenosella Wallker) is a new invasive alien pest in my country, belonging to Lepidoptera (Lepidoptera), Oecophoridae (Oecophoridae), coconut weaving moth English name coconut blackheaded caterpillar, Chinese translation has coconut blackhead caterpillar, leaf-eating caterpillar and coconut moth moth. Coconut weaver moth is an important pest on palm plants, and was included in the list of invasive alien pests by the State Forestry Administration in 2014. The insect originates in India, Sri Lanka and Myanmar, and seriously damages coconut trees. In recent years, the coconut weaver moth has spread to Thailand and Malaysia in the ASEAN countries, especially in Thailand, where the insect is seriously harmful. The pest fe...

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Problems solved by technology

[0004] There are also some defects in the above-mentioned technology, such as (1) the mating space of male and female adults is too small: in the prior art, each pair of male and female moths is separately placed in a glass tube with a length of 8.5 cm and a diameter of 4.5 cm to raise, but according to our research It was found that the adults of the coconut moth need a certain amount of space to mate. Before mating, the males need to perform a marriage flight "ceremony". It is also found that the coconut moth can mate with multiple males, thereby increasing the amount of eggs laid. However, in the prior art, one female and one male are paired, which seriously affects the mating probability of male and female worms and the amount of eggs laid by females.

(2) There are few places and small areas for females to lay eggs: in the prior art, two 8cm coconut leaves are placed in the glass tube where the male and female worms mate for females to lay eggs. Due to the small space of the test tube, the area of ​​the leaves is limited , which seriously affects the number of eggs laid by females, and the eggs on the leaves are seriously stacked, which greatly reduces the hatching rate of eggs

(3) The workload is heavy, the process is complicated, and the overall feeding efficiency is low: because the existing technology adopts single-tube feeding of male and female worms, it is necessary to replace the egg-laying leaves for each glass tube every day. Daily collection and replacement of spawning leaves will increase a lot of workload

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