Erythroid progenitor cell cryopreservation medium and its application

A technology of erythroid progenitor cells and cryopreservation solution, which is applied in the field of erythroid progenitor cell cryopreservation solution, can solve the problems of lack of cryopreservation research, and achieve the effects of good proliferation ability, good protection effect, and good regulation effect

Active Publication Date: 2016-06-29
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of research on cryopreservation for the specific cell type of erythroid progenitor cells

Method used

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  • Erythroid progenitor cell cryopreservation medium and its application
  • Erythroid progenitor cell cryopreservation medium and its application
  • Erythroid progenitor cell cryopreservation medium and its application

Examples

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Embodiment 1

[0034] The inventor adopts 4 kinds of cryopreservation solutions without plasma and 4 kinds of cryopreservation solutions containing plasma respectively to freeze the erythroid progenitor cells induced and expanded in vitro, and compares the cryopreservation effects in order to observe the effect of umbilical cord plasma on erythroid progenitor cells. Effects of cryopreservation of cells, and the possibility of reducing DMSO concentrations to reduce cytotoxicity. details as follows:

[0035] 1. Cryopreservation of erythroid progenitor cells in plasma-free cryopreservation medium

[0036] 1.1, isolate and obtain umbilical cord blood mononuclear cells, and adopt the Stemspan culture medium that has added SCF, IGF-1, EPO, transferrin and dexamethasone (wherein the concentration of SCF is 100ng / mL, and the concentration of IGF-1 is 40ng / mL, the concentration of EPO is 5U / mL, the concentration of transferrin is 100μg / mL, and the concentration of dexamethasone is 1μM), in T25 cell...

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Abstract

The invention discloses erythroid progenitor cell cryopreservation liquid and application thereof, wherein the erythroid progenitor cell cryopreservation liquid comprises: DMSO; HSA; and blood plasma. The cryopreservation solution can effectively preserve the erythroid progenitor cells, and has a long storage time, and the cells have strong proliferation ability after thawing, and the cell recovery rate is high.

Description

technical field [0001] The invention relates to the field of cell cryopreservation, especially the field of stem cell cryopreservation. Specifically, it relates to the field of cryopreservation of erythroid progenitor cells. More specifically, it relates to a cryopreservation solution for erythroid progenitor cells, a kit for cryopreserving erythroid progenitor cells and a method for cryopreserving erythroid progenitor cells. Background technique [0002] In recent years, due to the shortage of blood sources and the occurrence of blood transfusion-related diseases, people expect to find safer and sufficient blood sources. Studies have shown that stem cells can be induced to differentiate into red blood cells in vitro and can be used as a new blood source. In addition to mature erythrocytes, erythroid progenitor cells can also replace clinical erythrocyte transfusion and play a role in hematopoietic support therapy, and erythroid progenitor cells can continue to proliferate...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A01N1/02
Inventor陈琳裴雪涛习佳飞谢小燕周军年李艳华岳文房芳南雪刘大庆
OwnerFIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY