Application of solasodine in preparing antitumor drug

An anti-tumor drug, the technology of Australian solanamine, which is applied in the field of medicine, can solve the problems of regulation that have not been reported yet, and achieve the effects of overcoming drug resistance, enhancing the therapeutic effect, and reversing drug resistance characteristics

Inactive Publication Date: 2014-12-24
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] So far, there has been no report on the regulation of solanamine on the drug resistance of tumor cells

Method used

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  • Application of solasodine in preparing antitumor drug
  • Application of solasodine in preparing antitumor drug
  • Application of solasodine in preparing antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Implementation 1: CCK-8 kit to detect drug-resistant cell lines' resistance to solanamine

[0057] Experimental Materials:

[0058] Australian solanamine was purchased from the Institute of Materia Medica, Chinese Academy of Sciences, with a purity of not less than 98%. Human oral cancer cell line KB and its drug-resistant cell line KB / VCR were provided by the Chinese Academy of Sciences, human breast cancer cell line MCF-7 / ADR and its drug-resistant cell line MCF-7 / ADR, human colon cancer HCT-8 and The drug-resistant cell line HCT-8 / VCR was purchased from Nanjing Kaiji Biological Company. Verapamil (VPL), vincristine (VCR) and doxorubicin (ADR) were purchased from Roche Chemical Company with a purity greater than 99%. CCK-8 kit was purchased from Tongren Company.

[0059]

[0060] experimental method:

[0061] Cell recovery

[0062] 1) Take out the cryotube from the liquid nitrogen tank, put it directly into warm water at 37°C, and shake it from time to time to...

Embodiment 2

[0087] Example 2: CCK-8 method detects the reversal effect of solanamine on tumor cell multidrug resistance

[0088] Experimental material: with embodiment 1.

[0089] experimental method:

[0090] Solanamine sensitization experiment: KB / VCR, MCF-7 / ADR and HCT-8 / VCR cells were inoculated into 96-well plates at a density of 3500 / well, VCR with different concentrations after 24 hours, and VCR with different concentrations and Solanamine was mixed with α-MEM containing 10% fetal bovine serum and added to each well. After culturing for 48 h, the culture medium was discarded, and the CCK-8 kit was used to measure the activity of the drug-resistant cell line and its parental cells to VCR and VCR+Austhanamine in the same manner as in Example 1, and a curve was drawn. Three replicate wells were set for each concentration, and the experiment was repeated 3 times.

[0091]

[0092] Experimental results:

[0093] The multiplicity of drug resistance of KB / VCR cells to VCR was 81.9...

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Abstract

The invention belongs to the field of medicines, relates to application of solasodine in preparing an antitumor drug and particularly relates to application of solasodine in preparing a drug-resistant reversal agent of an antitumor drug. The solasodine is a natural product which has effect of reversing multidrug resistance of tumor cells, and can be used as a reversal agent for multidrug resistance of tumors. The solasodine further has effects of increasing sensitivity of tumor multidrug-resistant cells on the anti-tumor drug and can be used as a chemosensitizer. The invention further provides a method for inhibiting tumor multidrug-resistant cell multiplication by a pharmaceutical composition that combines the anti-tumor drug and solasodine. According to the invention, a small molecule compound solasodine can be used as a novel antitumor drug or an auxiliary ingredient thereof to be developed, and is obvious in tumor inhibiting effect, green and environment-friendly, so that a new way and means is provided for treating and curing tumors.

Description

technical field [0001] The invention belongs to the field of medicine, relates to reversing multi-drug resistance of tumors and sensitizing anti-tumor drugs, in particular to the application of solanamine in preparing tumor multi-drug reversing agents and anti-tumor drug sensitizers. Background technique [0002] Malignant tumors seriously endanger human life and health, and have become one of the main causes of death from diseases today. At present, the main treatment methods for malignant tumors include surgery, radiotherapy and chemotherapy. Chemotherapy is a better option for patients diagnosed with advanced tumors and those with metastases. Clinicians can optimize the maximum curative effect and minimum toxicity by adjusting the dosage of drugs, helping patients prolong survival time and improve the prognosis of life quality. However, the generation of drug resistance of tumor cells leads to a decrease in the sensitivity of tumor cells to chemotherapy drugs, which gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K31/58A61P35/00A61K31/475A61K31/337A61K31/513
Inventor 余龙朱恒锐
Owner FUDAN UNIV
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