Method for screening allelotype of immune related gene DQA1 Exon 2-(A-C) of goat

Abstract

The invention discloses a method for screening the allelotype of an immune related gene DQA1 Exon 2-(A-C) of goat. A primer is designed according to the sequence of exon-2 of a DQA1 gene, the genome DNA of white goat is used as a template to carry out PCR (Polymerase Chain Reaction) amplification, purification sequencing is carried out on the PCR product, and the polymorphic site of the exon-2 of DQA1 gene is screened preliminarily; a target fragment is subjected to PCR amplification by using DNA of the white goat genome as a template, and the PCR product is treated by a denaturing agent, and the denatured amplification fragment is subjected to polyacrylamidedel gel electrophoresis detection; rubber is dyed by applying a silver staining method, and partial allelotype of the exon-2 of the DQA1 gene of white goat from Guizhou is determined according to the result of polyacrylamidedel gel electrophoresis. A convenient, quick and accurate method is provided to PCR-SSCP screening and type determining of exon-2 polymorphism of DQA1 gene of different varieties of goat, and can be used for detecting partial allele of exon2 of goat immune related DQA1 gene, so that the problem of difficult type determination on highly polymorphic genes in the existing PCR-SSCP technology can be solved.

Description

technical field [0001] The invention relates to the goat molecular marker-assisted breeding technology in the field of animal biotechnology, and specifically designs a method for screening goat immune-related gene DQA1 Exon 2-(A-C) allele types. Background technique [0002] Guizhou white goat is an excellent local goat breed in Guizhou Province. It has the characteristics of strong adaptability, resistance to rough feeding, high fecundity, good fattening performance, good meat quality, and excellent cortex. It is suitable for grazing and breeding in mountains and hills. However, there are few domestic reports on the immune traits of Guizhou white goats. Therefore, how to use molecular biology techniques to quickly and efficiently screen and breed Guizhou white goats with high disease resistance has become the top priority for improving my country's excellent local goat breeds. Heavy. [0003] MHC (major histocompatibility complex, major histocompatibility complex) is a mult...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for screening goat immune-related gene DQA1 Exon 2-(A-C) allelic type, which is a PCR-SSCP screening and judgment method for the polymorphism of exon 2 of DQA1 gene in different varieties of goats.

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