Method for preparing receptor-ligand binding sulfonamide antibiotic protein

Abstract

The invention discloses a method for preparing a receptor-ligand binding sulfonamide antibiotic protein. The method comprises the following steps: firstly preparing a prokaryotic expression vector, and then isolating and purifying Escherichia coli strains to obtain dihydrofolate synthase; with a genome of Escherichia coli as a template, taking the obtained dihydrofolate synthetase gene fragment and cloning into the prokaryotic expression vector to obtain a cloning vector, and extracting recombinant plasmids from the cloning vector and the prokaryotic expression vector; culturing the recombinant plasmids and Escherichia coli in a culture medium to obtain a positive clone strain, and then continuously culturing the positive clone strain in the culture medium and centrifuging to obtain a thallus; concentrating the thallus and polyethylene glycol octylphenyl ether, and centrifuging to obtain a protein; and finally purifying the protein. According to the method for preparing the receptor-ligand binding sulfonamide antibiotic protein provided by the invention, a rapid detection product produced under the action of high binding force has sensitivity higher than that of the same product produced by an antibody binding principle.

Description

technical field [0001] The invention relates to a method for preparing receptor ligand-bound sulfonamide antibiotic protein. Background technique [0002] At present, there are various methods for detecting antibiotics in fresh milk, and the traditional methods include: microbiological detection, chromatography, immunology, etc. Although these traditional methods have high sensitivity, the detection steps are cumbersome, the requirements for equipment and operators are high, and it takes a long time, usually more than two hours. For enterprises, production efficiency is the first priority, so rapid detection is favored in enterprises. However, the current rapid detection kits are imported kits, and the cost is relatively high. The price of a kit for testing 96 samples is more than 3,000 yuan. This makes a small and medium-sized dairy company spend at least hundreds of thousands of funds in the field of antibiotic testing every year. The lack of domestic rapid and effectiv...

AI Technical Summary

Problems solved by technology

However, the current rapid detection kits are imported kits, and the cost is relatively high. The price of a kit for testing 96 samples is more than 3,000 yuan