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A method for making an animal model of primary dysmenorrhea of ​​cold coagulation and blood stasis type

A technology for primary dysmenorrhea and cold coagulation blood stasis, which is applied to the modeling of primary dysmenorrhea animal models of cold coagulation blood stasis type, and the application field of screening anti-cold coagulation blood stasis type primary dysmenorrhea drugs can solve problems such as limitations

Active Publication Date: 2017-04-12
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical evaluation and screening of drugs for primary dysmenorrhea of ​​anti-cold and blood stasis type are subject to certain restrictions

Method used

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  • A method for making an animal model of primary dysmenorrhea of ​​cold coagulation and blood stasis type

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. The method for making an animal model of primary dysmenorrhea of ​​cold coagulation and blood stasis type, including the following steps:

[0024] (1) Select female SD rats and place them in ice water for 5 consecutive days, then take them out and inject epinephrine hydrochloride, 0.16ml / rat / time, after an interval of 3 hours, inject epinephrine hydrochloride again, 0.16ml / rat / time , to produce cold-coagulation-blood-stasis rats;

[0025] (2) Then prepare the primary dysmenorrhea rat model with estrogen and oxytocin in combination, give the rats of the above step (1) subcutaneous injections of estradiol benzoate for 5 consecutive days, 1mL / kg / day, to synchronize Uterus, and the sensitivity of the uterus to oxytocin was improved. On the 6th day, rats were intraperitoneally injected with oxytocin, 1 mL / kg, to obtain a rat animal model of primary dysmenorrhea of ​​cold coagulation and blood stasis type.

Embodiment 2

[0027] 1. The method for making an animal model of primary dysmenorrhea of ​​cold coagulation and blood stasis type, including the following steps:

[0028] (1) Choose female SD rats and place them in ice water for 7 consecutive days, then take them out and inject epinephrine hydrochloride, 0.16ml / rat / time, after an interval of 4 hours, inject epinephrine hydrochloride again, 0.16ml / rat / time , to produce cold-coagulation-blood-stasis rats;

[0029] (2) Then prepare the primary dysmenorrhea rat model with estrogen and oxytocin in combination, give the rats of the above step (1) subcutaneous injections of estradiol benzoate for 7 consecutive days, 1mL / kg / day, to synchronize Uterus, and the sensitivity of the uterus to oxytocin was increased. On the 8th day, rats were intraperitoneally injected with oxytocin, 1 mL / kg, to obtain a rat animal model of primary dysmenorrhea of ​​cold coagulation and blood stasis type.

Embodiment 3

[0031] 1. Experimental grouping: Select 16 primary dysmenorrhea rats of the cold coagulation and blood stasis type obtained by modeling according to the method in Example 2 above, with a body weight of about 220 g, and divide them into a model group of 8 and an administration group (Shaofu Zhuyu Decoction) 8 rats, and 8 unmodeled rats were selected as the normal control group at the same time, with a body weight of about 220 g.

[0032] 2. Determination of biochemical indicators

[0033] Prostaglandins (PGs) are closely related to primary dysmenorrhea, PGF 2α It can stimulate uterine contraction and increase uterine tension. Studies have shown that the more severe the dysmenorrhea, the more PGF in the blood 2α The higher the content; oxytocin (oxytocin) oxytocin can act on uterine muscle cells to cause uterine contraction, and at the same time stimulate endometrial cells to release prostaglandins; neuroendocrine hormone β-endorphin is a neuroendocrine hormone related to pain;...

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Abstract

The invention discloses a model making method for a cold-coagulation blood-stasis type primary dysmenorrheal animal model. The model making method comprises the following steps: producing mice cold-coagulation blood-stasis by combining ice bath with epinephrine injection; then, preparing a primary dysmenorrheal mice model by combining estrogen with oxytocin, thereby obtaining the cold-coagulation blood-stasis type primary dysmenorrheal animal model. According to the theory of the traditional Chinese medicines and the pathogenesis of dysmenorrheal diseases, the mice cold-coagulation blood-stasis is produced by combining ice bath with epinephrine injection through large-scale experimental selection. The model making method for preparing mice cold-coagulation blood-stasis by combining ice bath with epinephrine injection is simple to operate, high in model making success rate, easy to popularize and apply, important in application value, and can be used for solving the defect that the cold-coagulation blood-stasis type primary dysmenorrheal animal model is absent at present, so that a novel method is provided for normative, standard and scientific traditional Chinese medicine evaluation.

Description

technical field [0001] The invention relates to a modeling method of an animal model, in particular to a modeling method of an animal model of primary dysmenorrhea of ​​cold coagulation blood stasis type and the application of the model in screening anti-cold coagulation blood stasis type primary dysmenorrhea drugs. Background technique [0002] The onset of dysmenorrhea has different causes such as emotional injury, careless daily life or harm caused by six evils, and is related to the fertile body, menstrual period, and the special physiological environment before and after menstruation. Its pathogenesis is mainly due to the influence of pathogenic factors during this period, resulting in chongren stasis or cold coagulation of the meridian, which makes the flow of qi and blood not smooth, and the circulation of uterine menstrual blood is hindered, resulting in "pain if no flow"; or chongren, The uterus is lost in nourishment, disgraceful and painful. The location of the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/12A61K49/00A61K31/137
Inventor 宿树兰段金廒黄晓晨郭建明刘培钱大玮
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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