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A kind of breeding method of all-fragrant soft rice two-line hybrid rice

A technology of hybrid rice and fragrant type, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of expanding genetic differences, poor quality, and inability to adapt to competition needs, etc.

Active Publication Date: 2018-02-13
文山壮族苗族自治州农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some defects in these fragrant hybrid rice breeding methods: first, the quality of the paired parents is not good, such as high amylose content, not soft rice type, although the paired breeding can obtain full-fragrant hybrid rice varieties or natural ginseng type However, due to the high content of amylose, the rice will regenerate after cold, harden and taste bad; the second is to match the parents, that is, the quality of the sterile line and the restorer line are inconsistent, such as the content of amylose is inconsistent , leading to hybrid rice (F 2 Genetic segregation of the quality traits of the generation seeds) results in the formation of miscellaneous rice with different hardness and softness, which is poor in quality and not easy to cook; thirdly, although both parents of the mating group have fragrance, the genes that control the fragrance are often unequal, and the hybrid mating group The hybrid rice variety obtained may not be a full-flavored variety with insufficient fragrance
However, compared with hybrid rice varieties, due to the lack of richer genetic basis of hybrid rice, the recombination of more excellent genes, and the expansion of genetic differences, the yield of conventional rice is always more than 20% lower than that of hybrid rice, resulting in a decline in rice production. Indica hybrid rice has high yield but poor quality, while high-quality fragrant soft rice conventional rice has good quality but low yield, which cannot meet the market's increasing requirements for rice quality, let alone adapt to the national food security strategy and The Competitive Demands of the International Market

Method used

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  • A kind of breeding method of all-fragrant soft rice two-line hybrid rice
  • A kind of breeding method of all-fragrant soft rice two-line hybrid rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 fragrance, the screening of soft rice gene donor variety material

[0045] The flow chart of the present invention carrying out the high-quality hybrid rice selection of full-flavor type soft rice with conventional breeding is as follows figure 1 As shown, the mark "can be the parents of each other" at the place of "fragrant soft rice variety I×rice two-line male sterile line II" means that the fragrant soft rice variety I can be used as the female parent, and the two lines of rice with different Breeding line II is the male parent hybrid, or the two-line male sterile line II of rice can be used as the female parent, and the fragrant soft rice variety I can be used as the male parent, and "fragrant soft rice variety V × restorer line IV" is hereby analogy.

[0046] If in combination with biotechnology, the high-quality hybrid rice breeding of full-flavored soft rice of the present invention is carried out, then it is: utilize conventional biotechnological ...

Embodiment 2

[0051] Example 2 Creation of Scented Soft Rice Two-line Sterile Wenxiang 12S

[0052] In this example, Dianrui 408 is used as the aroma and soft rice gene donor of the present invention——flavor type soft rice rice variety I, and the excellent two-line male sterile line Y58S is used as the nuclear male sterile gene donor material—two lines of rice CMS II, in accordance with figure 1 Breeding two-line CMS line III of fragrant soft rice, specifically:

[0053] In May 2009, Dianrui 408 was used as the female parent and Y58S was used as the male parent to carry out hybridization at the Sanya Experimental Base of Wenshan Academy of Agricultural Sciences, and the fragrance and soft rice genes were introduced into the excellent two-line sterile gene containing nuclear male sterile genes In the line, the fragrance, soft rice gene and nuclear male sterile gene are aggregated into one. Afterwards, according to the breeding selection goals of rice grains with fragrance, soft rice, slend...

Embodiment 3

[0057] Embodiment 3 Creation of Wenhui 968, the Restorative Line of Fragrant Soft Rice

[0058] In this embodiment, Hongyou 5 is used as the aroma and soft rice gene donor variety of the present invention——fragrant soft rice variety V, and Mianhui 725 is used as the strong restorer gene donor material——restorer line IV. figure 1 Breeding of fragrant soft rice restorer line VI, specifically:

[0059] In 2009, in the network room of Wenshan Academy of Agricultural Sciences, Mianhui 725, an excellent restorer line with no fragrance, high combining ability, strong resilience and wide adaptability, was used as the female parent, and Hongyou 5, an excellent fragrant soft rice variety, was used as the female parent. The male parent is crossed to integrate various excellent genes such as fragrance, soft rice, high combining ability, strong resilience, and wide adaptability. Afterwards, through continuous self-crossing and generation at the Wenshan and Sanya test bases of the Wenshan ...

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Abstract

The invention discloses a breeding method of fully-aromatic soft rice two-line hybrid rice. The breeding method specifically comprises the following steps: screening out an aromatic soft rice variety I and an aromatic soft rice variety V which have different genetic backgrounds and genotype of aabb, respectively importing the gene a, controlling aroma, and a gene b, with low-grain amylose content, in the aromatic soft rice variety I and the aromatic soft rice variety V into two-line sterile line II and a restoring line IV, and crossing the high-quality fully-aromatic soft two-line hybrid rice VII with the genotype of aabb by taking the obtained aromatic soft rice two-line sterile line III as a female parent and the aromatic soft rice restoring line VI as a male parent. According to the bred fully-aromatic soft rice two-line hybrid rice VII, the yield is high, each rice grain has aroma, and the rice is soft and cannot cause retrogradation after being cooled.

Description

technical field [0001] The invention relates to a hybrid rice breeding technology, in particular to a method for breeding two-line hybrid rice of whole-fragrant soft rice. Background technique [0002] The successful invention and application of indica hybrid rice breeding methods in my country has created huge economic and social benefits. However, hybrid rice is a segregated F 2 Generation seeds, the quality traits are between the parents, the combination of high-quality preparation of both parents, especially the allelism of high-quality trait genes such as fragrance genes, can fundamentally prevent F 2 Genetic segregation of rice grains, so that the internal quality of rice grains does not separate or the separation is controlled to a minimum. Since the physical and chemical indicators of the high-quality two-line hybrid rice parents in my country are all above the national excellent grade 3, it is difficult to prepare more high-quality hybrid rice combinations. As a r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 李存龙罗龙杨芬陶永宏韦永贵董卓娅张晓磊
Owner 文山壮族苗族自治州农业科学院
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