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Application of root bark extract of Actinidia sinensis in the preparation of anti-plant virus agent

An anti-plant virus agent, the technology of Chinese kiwifruit, which is applied in the fields of biological control chemicals, plant growth regulators, applications, etc., can solve problems such as unreported effects, and achieve broad-spectrum anti-plant virus activity and control effect. significant effect

Active Publication Date: 2017-12-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the effect of the root bark extract of Actinidia sinensis and its metabolites on agricultural production diseases, insects and weeds

Method used

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  • Application of root bark extract of Actinidia sinensis in the preparation of anti-plant virus agent
  • Application of root bark extract of Actinidia sinensis in the preparation of anti-plant virus agent
  • Application of root bark extract of Actinidia sinensis in the preparation of anti-plant virus agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the preparation of 20% kiwifruit root bark microemulsion

[0054] Weigh 20kg of kiwifruit root bark concentrate, dissolve it in 30kg of ethyl acetate, add 15kg of calcium dodecylbenzenesulfonate, 5kg of styrene acid polyoxyethylene ether, and 5kg of ethylene glycol, mix under high-speed stirring, and heat to about 40°C; add 25kg of deionized water dropwise under high-speed stirring, and control the dropping speed of the water to keep the temperature at about 40°C; Emulsion 100kg. The stability and appearance of the preparation meet the requirements of commercial preparations.

Embodiment 2

[0055] Embodiment 2: the preparation of 20% kiwi fruit root bark soluble liquid

[0056] Weigh 20kg of kiwifruit root bark concentrate, dissolve it in 30kg of ethyl acetate, add 5kg of alkylphenol polyoxyethylene ether, 1kg of alkyl polyglycoside A, and make up to 100kg with ethanol, mix well, and stir at a speed of 800~ Stir at 1000 rpm for 10 to 30 minutes to prepare 100 kg of kiwifruit root bark soluble liquid. The stability and appearance of the preparation meet the requirements of commercial preparations.

Embodiment 3

[0057] Embodiment 3: the preparation of 10% kiwifruit root bark water

[0058] Weigh 10kg of kiwifruit root bark concentrate, dissolve it in 30kg of ethyl acetate, then add 5kg of alkylphenol polyoxyethylene ether, 1kg of alkyl polyglycoside A, 5kg of ethylene glycol, make up to 100kg with water, mix well, Stirring at a stirring speed of 800-1000 rpm for 10-30 minutes can prepare 100 kg of kiwifruit root bark soluble liquid. The stability and appearance of the preparation meet the requirements of commercial preparations.

[0059] 5. Indoor bioassay of 3 kinds of kiwifruit root bark antiviral agents against TMV:

[0060] (1) In vitro passivation effect on TMV

[0061] The half-leaf dead spot method was used to test, and Nicotiana glutinosa at the 5-6 leaf stage, which grew vigorously and had the same growth vigor, was selected as the dead spot host. With the leaf veins as the boundary, the left half leaf was inoculated with an equal volume mixture of medicine and virus, and ...

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Abstract

The invention discloses the application of the root bark extract of Actinidia chinensis for preparing anti-plant virus agent. The root bark of Actinidia chinensis is taken, dried and crushed, and prepared into kiwifruit root bark antiviral soluble liquid and water agent through a certain processing process. and microemulsions, which can be used to prevent tobacco mosaic virus (TMV), cucumber mosaic virus (CMV) and potato virus (Potato Virus X, PVX; Potato Virus Y, PVY) and other plant virus diseases; the anti-plant virus agent of the present invention not only has good anti-virus effect, but also is safe to the environment, people, livestock, natural enemies of pests and other beneficial organisms. The preparation process of the anti-plant virus agent is simple, the cost is low, and it is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of plants and applications thereof, in particular to the application of the root bark extract of Actinidia sinensis for resisting plant viruses. Background technique [0002] Plant virus is an intracellular parasitic pathogen composed of nucleic acid and protein with infectious activity. According to the eighth report of the International Committee on Taxonomy of Viruses (ICTV), there are 1122 plant virus species found in the world. Plant diseases caused by plant viruses are known as "plant cancers". The annual loss caused by plant viruses in the world is about 40 billion U.S. dollars, and the damage caused by tobacco mosaic virus (TMV) alone exceeds 100 million U.S. dollars. [0003] Due to the strict parasitic nature of plant viruses, they parasitize in plant cells or even the nucleus to perform nucleic acid (RNA or DNA) replication and protein shell synthesis to form new virus particles. This determines that the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/08A01P1/00
Inventor 张秀云谢玉峰魏志平沈静吴华马志卿
Owner NORTHWEST A & F UNIV
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