Negative refractive index photonic crystal bioluminescence detector

Abstract

The invention discloses a negative refractive index photonic crystal bioluminescence detector. The bioluminescence detector comprises a main body box, an optical substrate and transparent colloid, wherein a negative refractive index photonic crystal in the main body box is formed by mutually alternating and overlapping a piezoelectric material layer and a negative refractive index material layer; the piezoelectric material layer is electrically connected with a control power supply; the narrow band transmission property of the negative refractive index photonic crystal is changed; the negative refractive index photonic crystal is adhered onto the optical substrate; a first photodetector and a second photodetector are arranged on the upper surface of the negative refractive index photonic crystal; the first photodetector and the second photodetector receive excited fluorescence signals filtered through the negative refractive index photonic crystal and convert the excited fluorescence signals into electrical signals; the electrical signals are transmitted to a processor, so as to be processed. The bioluminescence detector can realize the selection of excited laser wavelengths and filtering of fluorescence wavelengths through one device, can simultaneously detect various fluorescence tissues, solves the problem of quantitative calculation caused by different wavelengths, and ensures that the fluorescence measurement precision reaches 96.8 percent.

Description

technical field [0001] The invention relates to the technical field of biological tissue autofluorescence spectrum analysis, in particular to a negative refractive index photonic crystal bioluminescence detector. Background technique [0002] The fluorescence emission spectrum is the result of the fluorescent substance absorbing radiation and then emitting it. When the fluorescence excitation spectrum refers to the relative efficiency of emitting fluorescence of a certain wavelength caused by the excitation of different wavelengths, the fluorescence intensity is expressed by the integral area of ​​the fluorescence emission spectrum of the fluorescent substance The amount of fluorescence intensity. In recent years, with the emergence of different fluorescent markers, it has become possible to label different organelles in cells or detect different ions in cells. The appropriate excitation light source is selected according to the fluorescence emission characteristics of fluor...

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Problems solved by technology

In order to obtain the corresponding fluorescence characteristics, the above-mentioned invention patents all use an excitation light source with a single incident wavelength. This excitation light source cannot be adjusted, has a single function, and cannot identify multiple fluorescent tissues at the same time.

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