A Negative Refractive Index Photonic Crystal Type Bioluminescence Detector

Abstract

The invention discloses a negative refractive index photonic crystal type biological fluorescence detector, which comprises a main body box, an optical base and a transparent colloid, and the negative refractive index photonic crystal in the main body box is alternately overlapped by piezoelectric material layers and negative refractive index material layers Forming, the piezoelectric material layer is electrically connected to the control power supply, and the narrow-band transmission characteristics of the negative refractive index photonic crystal are changed. The negative refractive index photonic crystal is attached to the optical substrate, and the first photodetector and the negative refractive index photonic crystal are arranged on the upper surface of the negative refractive index photonic crystal. The second photodetector, the first photodetector and the second photodetector receive the excited fluorescent signal filtered by the negative refractive index photonic crystal, and convert the excited fluorescent signal into an electrical signal, and the electrical signal is sent to the processor for processing . The invention can realize the selection of the excitation laser wavelength and the filtering of the fluorescence wavelength with a single device, and can detect multiple fluorescent tissues at the same time, which solves the quantitative calculation problem caused by different wavelengths, and the fluorescence measurement accuracy reaches 96.8%.

Description

technical field [0001] The invention relates to the technical field of biological tissue autofluorescence spectrum analysis, in particular to a negative refractive index photonic crystal bioluminescence detector. Background technique [0002] The fluorescence emission spectrum is the result of the fluorescent substance absorbing radiation and then emitting it. When the fluorescence excitation spectrum refers to the relative efficiency of emitting fluorescence of a certain wavelength caused by the excitation of different wavelengths, the fluorescence intensity is expressed by the integral area of ​​the fluorescence emission spectrum of the fluorescent substance The amount of fluorescence intensity. In recent years, with the emergence of different fluorescent markers, it has become possible to label different organelles in cells or detect different ions in cells. The appropriate excitation light source is selected according to the fluorescence emission characteristics of fluor...

AI Technical Summary

Problems solved by technology

In order to obtain the corresponding fluorescence characteristics, the above-mentioned invention patents all use an excitation light source with a single incident wavelength. This excitation light source cannot be adjusted, has a single function, and cannot identify multiple fluorescent tissues at the same time.

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