Polypeptide available for specific binding to mycoplasma genitalium adhesin protein MgPa and application thereof

A technology of mycoplasma genitalium and adhesin, applied in the field of bioengineering, can solve problems such as drugs and vaccines that do not meet clinical needs

Inactive Publication Date: 2015-08-12
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The inventors discovered that certain proteins from bacteria called Actinomycetes have specific binding ability towards other organisms like mycobacteroides or pseudorabies causing disease such as chlamydial meningoencephalitis (CME). This makes them useful tools for developing new ways to fight against these infectious agents without affect their healthy flora.

Problems solved by technology

The technical problem addressed in this patents relating to mycobacterosis is how to effectively treat and protect against Mg contagions caused by sexual transmission due to menstruation disorders such as testicular germinary syndrome and syphilis fever; specifically targeted drug development towards specific targets within these organisms called transcription factor molecules involved in regulating important processes during virus entry into their respective organs.

Method used

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  • Polypeptide available for specific binding to mycoplasma genitalium adhesin protein MgPa and application thereof
  • Polypeptide available for specific binding to mycoplasma genitalium adhesin protein MgPa and application thereof
  • Polypeptide available for specific binding to mycoplasma genitalium adhesin protein MgPa and application thereof

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Embodiment Construction

[0014] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0015] The polypeptides provided in this example are screened from a phage display 12 peptide library with MgPa as the target molecule. The specific screening method does not need to be elaborated in the present invention, and the screened phages are now identified and analyzed. .

[0016] 1. Determination of phage peptide library titer

[0017] The phage peptide library was used in sterile LB liquid medium from 10 7 diluted to 10 14 Finally, the phage strain ER2738 in the logarithmic growth phase was mixed with each dilution of the phage and spread on a solid medium containing IPTG and X-gal for culture. Since the phage in the phage display library contains a unique lacZα gene, When the phage plaques grow on the culture plate containing IPTG and X-gal, the color is blue, while the...

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Abstract

The invention provides polypeptide available for specific binding to mycoplasma genitalium adhesin protein MgPa. An amino acid sequence of the polypeptide is shown in SEQ ID NO.1. DNA (deoxyribonucleic acid) of the polypeptide is inserted into a phage; after proliferation, whether the polypeptide is available for specific binding to the MgPa is determined via ELISA (enzyme-linked immuno sorbent assay). ELISA results show: the ability of a phage clone to bind to the MaPa is higher ( with OD450 value being higher than 1.5), and the phage is thus a positive phage clone; the polypeptide inserted into the surface of the phage can specifically recognize recombinant proteins of the MgPa and can specifically bind to them. The polypeptide can specifically bind to the mycoplasma genitalium adhesin protein MgPa, can be used in the preparation of vaccines for preventing mycoplasma genitalium infections and pharmaceuticals for treating reproductive system diseases caused by the mycoplasma genitalium infections, and is evidently effective.

Description

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Claims

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Application Information

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Owner NANHUA UNIV
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