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Gel drying device and gel drying method

A dry glue and gel technology, applied in the field of biochemistry, can solve the problems of complicated operation, fragmentation of protein glue, cracked glue, etc., and achieve the effects of solving difficult preparation, simple operation and simple production.

Inactive Publication Date: 2017-10-13
HUBEI UNIVERSITY OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the gels stored in the buffer are referenced or compared later, the gel operation is generally cumbersome and easily breaks the protein gel
There are also gel dry glue processing devices from abroad on the market. This dry glue device is expensive and cumbersome to operate. It is necessary to purchase a matching dry glue buffer, and the quality of the final dry glue cannot be guaranteed, and it is prone to cracking.

Method used

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  • Gel drying device and gel drying method
  • Gel drying device and gel drying method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Such as figure 1 As shown, the gel drying device provided by the present invention includes a first cover plate 10 and a bottom plate 30, the size of these two plates can be the same, and can be 20cm × 20cm × 3mm, and these two plates can be formed by two right-angled recesses. The shape card slot 40 is fixed. Specifically, the first cover plate 10 has an opening 11 at its center, and the size of the opening is 9cm×7cm. This gel dry gel device can be used for a dry gel of protein gel or DNA gel with a size of 5cm×8cm.

[0023] Further, the first cover plate 10 and the bottom plate 30 can be made of plexiglass plate or plastic material, and the right-angled concave slot 40 is made of plastic material such as PE, HDPE, PA or PP.

Embodiment 2

[0025] Such as figure 2 As shown, the gel drying device provided by the present invention includes a second cover plate 20 and a base plate 30, both of which are transparent plexiglass plates of 20cm × 20cm × 3mm, and these two plates can be made of two right-angle concave The card slot 40 is fixed. Specifically, the second cover 20 has an opening 21 at its center, and the size of the opening is 14cm×9cm. The gel dry gel device can be used for dry gel of two protein gels or DNA gels with a size of 5cm×8cm.

[0026] Further, the second cover plate 20 and the bottom plate 30 can be made of organic glass plate or plastic material.

[0027] A specific embodiment of the gluing method by means of the above-mentioned gel drying device will be described in detail below.

[0028] Example 1

[0029] 1. First prepare the acrylamide gel dry gel buffer:

[0030] 1. Reagents:

[0031] Methanol (analytical pure), acetic acid (analytical pure), glycerin (analytical pure), ultrapure wat...

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Abstract

The invention belongs to the technical field of biochemistry, and in particular relates to a gel drying device and a method for using the gel drying device. The slot is fixed; wherein the cover plate has an opening in the center. The gel drying device of the present invention is simple to manufacture and low in cost, and is used in conjunction with the drying gel buffer, and can be used for the preparation of dry gel of agarose gel or polypropylene amine gel; with the aid of the gel drying device of the present invention The dry glue method is easy to operate and effectively solves the current problems of difficult preparation of dry glue and inconvenient storage of biological samples.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a gel drying device and a gel drying method. Background technique [0002] In the process of molecular cloning, protein expression and identification, it is often necessary to electrophoresis DNA or protein with the help of agarose gel or polyacrylamide gel in order to accurately determine its molecular weight, and then carry out later DNA cutting gel recovery or Western blot and other experimental operations. The gel after electrophoresis is a very important and precious experimental data, which must be stored for a long time for later use. [0003] However, bacteria or fungi can grow if the gel is exposed to normal room temperature conditions. There are also cases where important protein gels are stored in ordinary pure water, protein electrophoresis buffer, or protein gel destaining solution, which can only preserve the protein gel for a short time. When th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N1/28
Inventor 李健阮绪芝李珊
Owner HUBEI UNIVERSITY OF MEDICINE