Detection and application of novel molecular marker IncRNA (long non-coding RNA) AB007962 for gastric cancer prognosis

A technology of lncrna-ab007962 and molecular markers, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve far-reaching clinical significance, high sensitivity, and good stability

Inactive Publication Date: 2015-11-04
王振宁
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a detection kit for a novel molecular marker of gastric cancer long-chain non-coding lncrna-AB007962, which ...

Method used

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  • Detection and application of novel molecular marker IncRNA (long non-coding RNA) AB007962 for gastric cancer prognosis
  • Detection and application of novel molecular marker IncRNA (long non-coding RNA) AB007962 for gastric cancer prognosis
  • Detection and application of novel molecular marker IncRNA (long non-coding RNA) AB007962 for gastric cancer prognosis

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Experimental program
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Embodiment 1

[0043] Preparation of the lncRNA-AB00762 detection kit (50 reactions) in the patient's gastric tissue includes:

[0044]

[0045] Detection steps of lncRNA-AB007962 expression in 96 pairs of gastric cancer and its paired adjacent normal tissues:

[0046] 1. Collection of clinical samples: 96 pairs of gastric cancer patients undergoing radical resection for gastric cancer archived during 2009-2010 were collected from the First Affiliated Hospital of China Medical University. The frozen fresh tissues of gastric cancer and their paired adjacent normal tissues were placed in 1.5 mlDNase / RNase-free (Axygen Company) centrifuge tube, transferred to a -80°C refrigerator through a liquid nitrogen bucket, and all patients had informed consent to participate in scientific research.

[0047] 2. Extraction of total RNA in tissue: Strictly follow the principle of operation on ice in a low temperature environment, take gastric cancer tissue and its paired adjacent normal tissue with a wei...

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Abstract

The invention discloses a detection kit and an application of a novel molecular marker IncRNA (long non-coding RNA) AB007962 for gastric cancer prognosis and provides the molecular marker IncRNA AB007962 with down-regulated expression in the tissue of patients with gastric cancer. Prognosis of patients with low IncRNA AB007962 expression with the Lauren type being intestinal type is poorer than that of patients with high IncRNA AB007962 expression, when the IncRNA AB007962 expression is applied to prognosis prediction of patients with gastric cancer with the Lauren type being intestinal type, powerful molecular biological foundation can be provided for prognosis of the patients, and the detection kit and the application have great clinical meaning and broad application prospects.

Description

Technical field: [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the field of prognosis after radical gastrectomy. Specifically, the invention relates to the use of a novel molecular marker lncRNA-AB007962 for prognosis of gastric cancer and a detection kit prepared therefor. Background technique: [0002] Gastric cancer is one of the most common malignant tumors in the world. In my country, the morbidity and mortality of gastric cancer rank among the top. Because of the atypical early symptoms, most of them have reached the advanced stage when they are diagnosed clearly. Gastric cancer patients can be divided into various types according to different principles. For example, according to the degree of tissue differentiation, they can be divided into high, medium, and poorly differentiated gastric cancer. In addition, Lauren's classification, as a frequently used classification method in clinical practice, can classify gastric...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q1/6851C12Q2600/118C12Q2600/158C12Q2600/178C12Q2531/113C12Q2545/101C12Q2561/101C12Q2563/107
Inventor 王振宁宋永喜王嘉俊马斌杨宇翀孙景旭高鹏陈晓婉
Owner 王振宁
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